Fluorescent probe strategy for live cell distinction

Live cell discrimination is the first and essential step to understand complex biosystems. Conventional cell discrimination involving various antibodies relies on selective surface biomarkers. Compared to antibodies, the fluorescent probe strategy allows the utilisation of intracellular biomarkers,...

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Published inChemical Society reviews Vol. 51; no. 5; pp. 1573 - 1591
Main Authors Liu, Xiao, Chang, Young-Tae
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 07.03.2022
Subjects
Antibodies
Biomarkers
Biomolecules
Carbohydrates
Discrimination
Fluorescent Dyes - chemistry
Fluorescent indicators
Lipids
Metabolism
Proteins
Selective surfaces
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Summary:Live cell discrimination is the first and essential step to understand complex biosystems. Conventional cell discrimination involving various antibodies relies on selective surface biomarkers. Compared to antibodies, the fluorescent probe strategy allows the utilisation of intracellular biomarkers, providing broader options with unique chemical principles to achieve the live cell distinction. In general, fluorescent probes can be retained in cells by interacting with biomolecules, accumulating via transporters, and participating in metabolism. Based on the target difference, fluorescent probe strategy can be divided into several categories: protein-oriented live cell distinction (POLD), carbohydrate-oriented live cell distinction (COLD), DNA-oriented live cell distinction (DOLD), gating-oriented live cell distinction (GOLD), metabolism-oriented live cell distinction (MOLD) and lipid-oriented live cell distinction (LOLD). In this review, we will outline the concepts and mechanisms of different strategies, introduce their applications in cell-type discrimination, and discuss their advantages and challenges in this area. We expect this tutorial will provide a new perspective on the mechanisms of fluorescent probe strategy and facilitate the development of cell-type-specific probes. This tutorial review outlines the concepts and mechanisms of different fluorescent probe strategies for live cell distinction, introduces their applications in cell-type discrimination, and discusses their advantages and challenges in this area.
Bibliography:Xiao Liu graduated from Soochow University (China) with a BSc in 2017. He joined Prof. Young-Tae Chang's research group at the National University of Singapore in 2016 and moved to Pohang University of Science and Technology (POSTECH, Korea) as a graduate student in 2017. His research interests are the development of new fluorescent molecular rotors for bioimaging.
Young-Tae Chang studied chemistry at Pohang University of Science and Technology (POSTECH, Korea) and received his BS in 1991 and PhD in 1997 (Advisor: Prof. Sung-Kee Chung). He did his postdoctoral work with Prof. Peter Schultz at UC Berkeley and The Scripps Research Institute. In 2000, he started his academic career at New York University and in 2007, he moved to the National University of Singapore and Singapore Bioimaging Consortium. In 2017, he moved back to POSTECH as a faculty member and associate director of the Center for Self-assembly and Complexity, IBS. He has published more than 370 scientific papers and filed 50 patents so far. The full list is available at
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http://ytchang.postech.ac.kr
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ISSN:0306-0012
1460-4744
DOI:10.1039/d1cs00388g