Sequencing of cDNA clones from the genetic map of tomato (Lycopersicon esculentum)

• Published in: Genome research Vol. 8; no. 8; pp. 842 - 847
• Main Authors: Ganal, M W, Czihal, R, Hannappel, U, Kloos, D U, Polley, A, Ling, H Q
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.08.1998
• Subjects: Arabidopsis - genetics; Chromosome Mapping; Cloning, Molecular; Databases, Factual; DNA, Complementary; DNA, Plant - genetics; Genes, Plant; Genetic Markers; Letters; Lycopersicon esculentum; Lycopersicon esculentum - genetics; Molecular Sequence Data; Plant Proteins - chemistry; Plant Proteins - genetics; Polymorphism, Restriction Fragment Length; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Sequence Tagged Sites; Transcription, Genetic
• ISSN: 1088-9051; 1549-5469
• DOI: 10.1101/gr.8.8.842

The dense RFLP linkage map of tomato (Lycopersicon esculentum) contains >300 anonymous cDNA clones. Of those clones, 272 were partially or completely sequenced. The sequences were compared at the DNA and protein level to known genes in databases. For 57% of the clones, a significant match to previously described genes was found. The information will permit the conversion of those markers to STS markers and allow their use in PCR-based mapping experiments. Furthermore, it will facilitate the comparative mapping of genes across distantly related plant species by direct comparison of DNA sequences and map positions. [cDNA sequence data reported in this paper have been submitted to the EMBL database under accession nos. AA824695-AA825005 and the dbEST_Id database under accession nos. 1546519-1546862.]