Responses of Halobacterium halobium and Sulfolobus solfataricus to hydrogen peroxide and N-methyl-N-nitrosoguanidine exposure

• Published in: Microbiological research Vol. 152; no. 3; pp. 257 - 260
• Main Authors: Praul, Craig A., Taylor, William D.
• Format: Journal Article
• Language: English
• Published: Basingstoke Elsevier GmbH 01.09.1997; Nature Publishing Group
• Subjects: AP Endonuclease; Archaea; Bacteriology; Biological and medical sciences; DNA Repair; Fundamental and applied biological sciences. Psychology; Genetics; Halophile; Microbiology; Molecular and cellular biology; Molecular genetics; Mutagenesis. Repair; Thermophile; AP Endonuclease; Halophile; Thermophile; Archaea; DNA Repair; Archaeobacteria; Thermophily; Sulfolobus solfataricus; Mutagen; Hydrogen Peroxides; Sulfolobaceae; Halobacterium salinarum; Halophily; Halobacteriales; DNA; Bacteria; Sulfolobales; Halobacteriaceae; Repair
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/S0944-5013(97)80037-7

We have examined the capacity of Halobacterium halobium and Sulfolobus solfataricus to withstand the lethal effects of hydrogen peroxide and N-methyl-N-nitrosoguanidine. We tested a variety of pretreatment regimens with both mutagens and all failed to elicit an inducible response to the lethal effects of those compounds in either organism. We have observed AP endonuclease activity in protein extracts from both organisms. In addition, S. solfataricus extracts contain activities that remove 3-methyl-adenine and O 6-methyl-guanine from methylated DNA. This is the first report of these DNA repair activities in any member of the Archaea.