Spectrofluorimetric determination of hydrogen peroxide scavenging activity

• Published in: Analytica chimica acta Vol. 452; no. 2; pp. 177 - 184
• Main Authors: Paździoch-Czochra, Marzanna, Wideńska, Anna
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 11.02.2002; Elsevier
• Subjects: Analytical chemistry; Antioxidant; Chemistry; Exact sciences and technology; H 2O 2 scavenging ability; Herbs; Homovanillic acid; Phenolic compounds; Spectrofluorimetry; Spectrometric and optical methods; Herbs; H 2O 2 scavenging ability; Spectrofluorimetry; Antioxidant; Phenolic compounds; Homovanillic acid; Hydrogen peroxide; Enzyme; Scavenging; Fluorescence spectrometry; Peroxidases; H2O2 scavenging ability; Oxidation; Peroxidase; Oxidoreductases
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/S0003-2670(01)01455-6

Homovanillic acid (HVA) is widely used for the detection and imaging of oxidative enzymes—peroxidase, glucose oxidase and xanthine oxidase, but antioxidant activity has not been determined so far with the use of HVA. We have developed a simple, sensitive and in-field spectrofluorimetric method for the determination of hydrogen peroxide (H 2O 2) scavenging activity. The assay is based on the oxidation of HVA to its fluorescent biphenyl dimer in the presence of H 2O 2 and peroxidase. The presence of substances with H 2O 2 scavenging activity prevents the oxidation of HVA by removing H 2O 2. The decrease in fluorescence intensity is proportional to the antioxidative (H 2O 2 scavenging) activity. The method was evaluated using Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), BHA (3- t-butyl-4-hydroxyanisole) and ferulic, vanillic, caffeic, chlorogenic, protocatechuic and oxalic acids. Additionally, tea and herb infusions known for their antioxidant properties were evaluated.