Characterisation of cell movement by impedance measurement on fibroblasts grown on perforated Si-membranes

Mouse fibroblasts grown on perforated Si-membranes (pore diameter ∼ 10 μm have been studied to clarify cell locomotive ability. The cell motility was microscopically monitored by a time-lapse video system and, simultaneously, the impedance of the growing cells was measured every 5 s. The correlation...

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Published inBiochimica et biophysica acta Vol. 1269; no. 3; pp. 221 - 232
Main Authors Hagedorn, Rolf, Fuhr, Günter, Lichtwardt-Zinke, Konstanze, Richter, Ekkehard, Hornung, Jan, Voigt, Andreas
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 30.11.1995
Subjects
3T3 Cells
Animals
Cell Division
Cell motility
Cell Movement
Cell Size
Electrophysiology
Fibroblast
Fibroblasts - cytology
Impedance
Membranes, Artificial
Mice
Microscopy, Electron, Scanning
Pore
Silicon
Silicon membrane
Cell motility
Pore
Impedance
Silicon membrane
Fibroblast
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Summary:Mouse fibroblasts grown on perforated Si-membranes (pore diameter ∼ 10 μm have been studied to clarify cell locomotive ability. The cell motility was microscopically monitored by a time-lapse video system and, simultaneously, the impedance of the growing cells was measured every 5 s. The correlations between observed cell activities and measured impedance events are discused and classified. The methods is sensitive and allows discrimination between signals arising from translocation of single cells and those arising from filopodia activities. Both cell and filopodia motion could be detected. Designs of microdevices fabricated in semiconductor technology are presented.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0167-4889
0006-3002
1879-2596
DOI:10.1016/0167-4889(95)00122-4