Interaction of Pseudostellaria heterophylla with Fusarium oxysporum f.sp. heterophylla mediated by its root exudates in a consecutive monoculture system

• Published in: Scientific reports Vol. 5; no. 1; p. 8197
• Main Authors: Zhao, Yongpo, Wu, Linkun, Chu, Leixia, Yang, Yanqiu, Li, Zhenfang, Azeem, Saadia, Zhang, Zhixing, Fang, Changxun, Lin, Wenxiong
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 03.02.2015
• Subjects: Acids; Allelochemicals; Caryophyllaceae - chemistry; Caryophyllaceae - metabolism; Caryophyllaceae - microbiology; Chromatography, High Pressure Liquid; Exudates; Fusarium - genetics; Fusarium - growth & development; Fusarium - physiology; High-performance liquid chromatography; Hydroxybenzoates - analysis; Phenolic acids; Plant Exudates - chemistry; Plant Exudates - metabolism; Plant Roots - chemistry; Plant Roots - metabolism; Plant Roots - microbiology; Polymerase chain reaction; Real-Time Polymerase Chain Reaction; Replant disease; Rhizosphere; Rhizosphere microorganisms; RNA, Fungal - analysis; Secretion; Soil microorganisms; Spores; Spores, Fungal - growth & development
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep08197

In this study, quantitative real-time PCR (qPCR) was used to determine the amount of Fusarium oxysporum, an important replant disease pathogen in Pseudostellaria heterophylla rhizospheric soil. Moreover, HPLC was used to identify phenolic acids in root exudates then it was further to explore the effects of the phenolic acid allelochemicals on the growth of F. oxysporum f.sp. heterophylla. The amount of F. oxysporum increased significantly in P. heterophylla rhizosphere soil under a consecutive replant system as monitored through qPCR analysis. Furthermore, the growth of F. oxysporum f.sp. heterophylla mycelium was enhanced by root exudates with a maximum increase of 23.8%. In addition, the number of spores increased to a maximum of 12.5-fold. Some phenolic acids promoted the growth of F. oxysporum f.sp. heterophylla mycelium and spore production. Our study revealed that phenolic acids in the root secretion of P. heterophylla increased long with its development, which was closely related to changes in rhizospheric microorganisms. The population of pathogenic microorganisms such as F. oxysporum in the rhizosphere soil of P. heterophylla also sharply increased. Our results on plant-microbe communication will help to better clarify the cause of problems associated with P. heterophylla under consecutive monoculture treatment.