Mir-454-3p induced WTX deficiency promotes hepatocellular carcinoma progressions through regulating TGF-β signaling pathway

• Published in: Journal of Cancer Vol. 13; no. 6; pp. 1820 - 1829
• Main Authors: Liu, Hui, Zheng, Juan, Yang, Shengqian, Zong, Qibei, Wang, Zhiwen, Liao, Xinghua
• Format: Journal Article
• Language: English
• Published: Australia Ivyspring International Publisher Pty Ltd 01.01.2022; Ivyspring International Publisher
• Subjects: Autophagy; Cell growth; Liver cancer; Medical diagnosis; Medical prognosis; Plasmids; Protein expression; Proteins; Research Paper; Statistical analysis; Survival analysis; Tumors; United States > US; China; miR-454-3p; HCC; WTX; autophagy; metastasis
• ISSN: 1837-9664; 1837-9664
• DOI: 10.7150/jca.67478

Wilms tumor gene on X chromosome (WTX) is an X-linked tumor suppressor gene in Wilms tumor; however, however, the molecular mechanism of WTX in the occurrence and development of HCC has not been reported. The expression of miR-454-3p and WTX wre analyzed in 32 matched human HCC and normal tissue samples. The molecular mechanisms of miR-454-3p/WTX/TGFβ signaling in cell proliferation, migration, invasion and autophagy were investigated and . WTX expression was downregulated in HCC tissues; lower WTX levels were associated with poor HCC patient outcomes. WTX loss triggers the activation of TGF-β signaling, which promotes HCC cells proliferation, migration, invasion and autophagy. Further mechanistic study showed that the aberrant upregulation of miR-454-3p was identified as the reason of WTX loss in HCC. WTX is a tumor suppressor gene in HCC, miR-454-3p/WTX/TGFβ signaling will provide a new direction for the diagnosis and treatment of HCC.