Determination of cell metabolite VEGF165 and dynamic analysis of protein–DNA interactions by combination of microfluidic technique and luminescent switch-on probe

• Published in: Biosensors & bioelectronics Vol. 79; pp. 41 - 47
• Main Authors: Lin, Xuexia, Leung, Ka-Ho, Lin, Ling, Lin, Luyao, Lin, Sheng, Leung, Chung-Hang, Ma, Dik-Lung, Lin, Jin-Ming
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.05.2016
• Subjects: Aptamers, Nucleotide - chemistry; Biosensing Techniques - methods; Biosensors; Cell Line; Coordination Complexes - chemistry; Design analysis; Functional nucleic acid; G-Quadruplexes; Humans; Hydrodynamics; Iridium (III) probe; Iridium - chemistry; Kinetic analysis; Limit of Detection; Luminescence; Luminescent Agents - chemistry; Luminescent Measurements - methods; Metabolism; Metabolites; Microfluidic Analytical Techniques - methods; Microfluidic device; Microfluidics; Quantitative analysis; Recognition; Vascular Endothelial Growth Factor A - analysis; VEGF165; Kinetic analysis; Iridium (III) probe; VEGF165; Functional nucleic acid; Microfluidic device; VEGF
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2015.11.089

In this paper, we rationally design a novel G-quadruplex-selective luminescent iridium (III) complex for rapid detection of oligonucleotide and VEGF165 in microfluidics. This new probe is applied as a convenient biosensor for label-free quantitative analysis of VEGF165 protein from cell metabolism, as well as for studying the kinetics of the aptamer–protein interaction combination with a microfluidic platform. As a result, we have successfully established a quantitative analysis of VEGF165 from cell metabolism. Furthermore, based on the principles of hydrodynamic focusing and diffusive mixing, different transient states during kinetics process were monitored and recorded. Thus, the combination of microfluidic technique and G-quadruplex luminescent probe will be potentially applied in the studies of intramolecular interactions and molecule recognition in the future. Kinetics study of the interaction of protein and DNA on a microfluidic device and determination of VEGF165 from cell metabolism using a switch-on iridium probe. [Display omitted] •A rapid mixing microfluidic device was designed from milliseconds to minutes.•Dynamic monitoring to aptamer–protein interactions on a microfluidic device.•Detection of VEGF165 using a highly selective luminescent switch-on probe.