MiR-30a-5p promotes cholangiocarcinoma cell proliferation through targeting SOCS3

• Published in: Journal of Cancer Vol. 11; no. 12; pp. 3604 - 3614
• Main Authors: Zhang, Jia Wei, Wang, Xing, Li, Gao Chao, Wang, Dong, Han, Sheng, Zhang, Yao Dong, Luo, Chen Huan, Wang, Hong Wei, Jiang, Wang Jie, Li, Chang Xian, Li, Xiang Cheng
• Format: Journal Article
• Language: English
• Published: Australia Ivyspring International Publisher Pty Ltd 01.01.2020; Ivyspring International Publisher
• Subjects: Apoptosis; Bile ducts; Cell growth; Cholangiocarcinoma; Liver cancer; Metastasis; Research Paper; Software; Tumorigenesis; Tumors; United States > US; China; Japan; SOCS3; apoptosis; proliferation; miR-30a-5p; cholangiocarcinoma
• ISSN: 1837-9664; 1837-9664
• DOI: 10.7150/jca.41437

: MicroRNAs (miRNAs) play important roles in the occurrence and development of cancers. In this project, we aimed to explore the role and molecular mechanism of mir-30a-5p in cholangiocarcinoma (CCA). : The expression profile and clinical significance of miR-30a-5p in CCA patients were investigated in 31 ICC and 52 ECC patients respectively. The role and mechanism of miR-30a-5p in CCA cells were investigated by up-regulating and inhibiting miR-30a-5p expression functional study. : The expression of miR-30a-5p was increased in both CCA tissues and cells. The inhibition of miR-30a-5p decreased cell proliferation and induced cell apoptosis while overexpression of miR-30a-5p achieved the opposite effect. Furthermore, SOCS3 was down-regulated in ICC and ECC tissues and negatively regulated by miR-30a-5p. Dual-luciferase reporter assay revealed that co-transfection of miR-30a-5p significantly inhibited the activity of firefly luciferase reporter carrying the wild-type 3'UTR of SOCS3. The inhibition of SOCS3 could largely rescue the inhibitory effect of miR-30a-5p inhibition on CCA cells proliferation. In clinical, up-regulated miR-30a-5p expression was correlated with large tumor size in both ICC and ECC cohorts. : miR-30a-5p promoted CCA cells proliferation through targeting SOCS3. These findings suggested that miR-30a-5p could be a potential therapeutic target.