The revelation of glucose adsorption mechanisms on hierarchical metal-organic frameworks using a surface plasmon resonance sensor

• Published in: Journal of materials chemistry. B, Materials for biology and medicine Vol. 11; no. 2; pp. 4428 - 4444
• Main Authors: Gumilar, Gilang, Chowdhury, Silvia, Shukri, Ganes, Patah, Aep, Nugraha, Nugraha, Henzie, Joel, Anshori, Isa, Kaneti, Yusuf Valentino, Yuliarto, Brian
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 24.05.2023
• Subjects: Adsorption; Ascorbic acid; Biomolecules; Biosensing Techniques; Biosensors; Copper; Glucose; Gold; Maltose; Manganese; Metal-Organic Frameworks; Nickel; Receptor mechanisms; Receptors; Resonance; Sensitivity; Surface chemistry; Surface plasmon resonance; Surface Plasmon Resonance - methods; Urea; Uric acid; Zirconium
• ISSN: 2050-750X; 2050-7518; 2050-7518
• DOI: 10.1039/d3tb00138e

The gold layer on the surface plasmon resonance (SPR) sensor chip cannot detect small molecules, such as glucose without the use of specific receptors. Metal-organic frameworks (MOFs) are useful in biosensing technologies for capturing and co-localizing enzymes and receptors with the target biomolecule. In many previous studies, the properties of the MOFs were often ignored, with these studies focusing on the selection of appropriate receptors. To take advantage of the unique properties of MOFs in biosensors, one must also consider the technique and transducer used because these aspects will strongly influence the detection mechanism. In this work, we have investigated for the first time, the applications of hierarchical metal-BDC (M-BDC) MOFs for glucose detection using the SPR technique without the use of specific receptors. The underlying interactions and adsorption mechanisms were analyzed using adsorption isotherm and kinetic models. The sensing measurements show that the SPR chips functionalized with M-BDC MOFs exhibit higher sensitivity and lower limit of detection (LOD). Specifically, the sensitivity follows the order of Zr-BDC > Cu-BDC > Mn-BDC > Ni-BDC > bare Au SPR chips with the LOD in the order of Zr-BDC < Mn-BDC < Ni-BDC < Cu-BDC < bare Au SPR chips. The selectivity test results reveal that Zr-BDC exhibits a decent selectivity to glucose in the presence of other interfering compounds, such as ascorbic acid, uric acid, maltose, and urea. These results demonstrate the promising potential of MOFs for SPR biosensing. This work reports the use of M-BDC (M = Cu, Mn, Ni, and Zr) MOFs for glucose sensing by surface plasmon resonance (SPR) methods. The sensitivity is in the order of Zr-BDC > Cu-BDC > Mn-BDC > Ni-BDC > bare Au chip.