Distinct transcriptional programs define a heterogeneous neuronal ensemble for social interaction

Social interactions are encoded by the coordinated activity of heterogeneous cell types within distributed brain regions including the medial prefrontal cortex (mPFC). However, our understanding of the cell types which comprise the social ensemble has been limited by available mouse lines and relian...

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Bibliographic Details
Published iniScience Vol. 27; no. 7; p. 110355
Main Authors Walker, Hailee, Frost, Nicholas A.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 19.07.2024
Elsevier
Subjects
Behavioral neuroscience
Cognitive neuroscience
Molecular neuroscience
Neuroscience
Omics
Molecular neuroscience
Neuroscience
Cognitive neuroscience
Behavioral neuroscience
Omics
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Summary:Social interactions are encoded by the coordinated activity of heterogeneous cell types within distributed brain regions including the medial prefrontal cortex (mPFC). However, our understanding of the cell types which comprise the social ensemble has been limited by available mouse lines and reliance on single marker genes. We identified differentially active neuronal populations during social interactions by quantifying immediate-early gene (IEG) expression using snRNA-sequencing. These studies revealed that distinct prefrontal neuron populations composed of heterogeneous cell types are activated by social interaction. Evaluation of IEG expression within these recruited neuronal populations revealed cell-type and region-specific programs, suggesting that reliance on a single molecular marker is insufficient to quantify activation across all cell types. Our findings provide a comprehensive description of cell-type specific transcriptional programs invoked by social interactions and reveal insights into the neuronal populations which compose the social ensemble. [Display omitted] •Heterogeneous neuronal populations are recruited during social interaction•Excitatory and inhibitory populations are variably recruited in the mPFC•Active neuronal populations were identified by quantifying IEG expression•Cell-type and region-specific IEG transcriptional programs were observed Neuroscience; Behavioral neuroscience; Molecular neuroscience; Cognitive neuroscience; Omics; social interaction, immediate early genes, medial prefrontal cortex, single cell
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ISSN:2589-0042
2589-0042
DOI:10.1016/j.isci.2024.110355