Comparison of three serological methods for detection of Lewis antigens on the surface of Helicobacter pylori

• Published in: FEMS microbiology letters Vol. 190; no. 1; pp. 67 - 72
• Main Authors: Hynes, Sean O, Moran, Anthony P
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.09.2000; Blackwell; Oxford University Press
• Subjects: Antigens; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Blood groups; Blotting, Western - methods; Clinical isolates; Electrophoresis, Polyacrylamide Gel - methods; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Fundamental and applied biological sciences. Psychology; Helicobacter Infections - microbiology; Helicobacter pylori; Helicobacter pylori - immunology; Helicobacter pylori - isolation & purification; Helicobacter pylori - metabolism; Humans; Immunoblotting; Lewis antigen; Lewis antigens; Lewis Blood-Group System - analysis; Lipopolysaccharide; Lipopolysaccharides; Lipopolysaccharides - analysis; Microbiology; Organic chemistry; Pathogenesis; Reference Standards; Virulence; Western blotting; Lipopolysaccharide; Immunoblotting; Lewis antigen; Virulence; Enzyme-linked immunosorbent assay; Spirillales; Spirillaceae; Method; Enzyme immunoassay; Antigen; Lewis system; Helicobacter pylori; Serological method; Immunoblotting assay; Bacteria; Detection; ELISA assay; Comparative study
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2000.tb09264.x

Abstract Chemical studies have shown that lipopolysaccharide (LPS) from certain strains of Helicobacter pylori expresses the Lewis (Le) blood group antigens, predominantly Lex and/or Ley. Serological methods have been used in a number of studies to assess the level of Le expression by H. pylori. However, serological and chemical analyses of a strain's LPS do not always agree. The purpose of this study was to evaluate and compare serological methods used to detect the expression of these antigens by H. pylori. A set of 34 Irish clinical isolates and three culture collection strains had their Le antigen expression assessed using three serological methods, serodot, Western blotting and enzyme-linked immunosorbent assay. A combination of all three methods was used to estimate Lex (59%) and Ley (94%) expression and this may allow a more accurate assessment of Le expression by isolates for pathogenesis studies.