Permanent proteins in the urine of healthy humans during the Mars-500 experiment

• Published in: Journal of bioinformatics and computational biology Vol. 13; no. 1; p. 1540001
• Main Authors: Larina, Irina M, Pastushkova, Lyudmila Kh, Tiys, Evgeny S, Kireev, Kirill S, Kononikhin, Alexey S, Starodubtseva, Natalia L, Popov, Igor A, Custaud, Marc-Antoine, Dobrokhotov, Igor V, Nikolaev, Evgeny N, Kolchanov, Nikolay A, Ivanisenko, Vladimir A
• Format: Journal Article
• Language: English
• Published: Singapore 01.02.2015
• Subjects: Adult; Aerospace Medicine - methods; Blood Proteins - analysis; Blood Proteins - metabolism; Healthy Volunteers; Humans; Liver - metabolism; Male; Mass Spectrometry - methods; Proteins - analysis; Proteins - metabolism; Proteinuria - diagnosis; Proteomics - methods; Social Isolation; Space Flight; Urinary proteome; associative gene networks; constant subproteome of urine; chromatography-mass spectrometry
• ISSN: 1757-6334
• DOI: 10.1142/S0219720015400016

Urinary proteins serve as indicators of various conditions in human normal physiology and disease pathology. Using mass spectrometry proteome analysis, the permanent constituent of the urine was examined in the Mars-500 experiment (520 days isolation of healthy volunteers in a terrestrial complex with an autonomous life support system). Seven permanent proteins with predominant distribution in the liver and blood plasma as well as extracellular localization were identified. Analysis of the overrepresentation of the molecular functions and biological processes based on Gene Ontology revealed that the functional association among these proteins was low. The results showed that the identified proteins may be independent markers of the various conditions and processes in healthy humans and that they can be used as standards in determination of the concentration of other proteins in the urine.