Significance of selenium supplementation in root- shoot reactions under manganese stress in wheat seedlings – biochemical and cytological studies

• Published in: Plant and soil Vol. 468; no. 1-2; pp. 389 - 410
• Main Authors: Sieprawska, Apolonia, Skórka, Magdalena, Bednarska-Kozakiewicz, Elżbieta, Niedojadło, Katarzyna, Janiak, Agnieszka, Telk, Anna, Filek, Maria
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.11.2021; Springer; Springer Nature B.V
• Subjects: Agricultural practices; Agriculture; Analysis; Biomedical and Life Sciences; Cell differentiation; Cytology; DNA methylation; Ecology; Hydrogen peroxide; Hydroponics; Identification and classification; Ions; Leaves; Life Sciences; Lipid peroxidation; Lipids; Manganese; Measurement; Meristems; Methods; Methylation; Nutrients; Peroxidation; Plant Physiology; Plant Sciences; Regular Article; Roots; Roots (Botany); Seedlings; Selenium; Shoots (Botany); Soil Science & Conservation; Stress; Supplements; Wheat; Poland; DNA methylation; Manganese stress; Selenium; Wheat seedlings
• ISSN: 0032-079X; 1573-5036
• DOI: 10.1007/s11104-021-05138-x

Purpose Agronomic practices are one of the reasons for the increasing accumulation of elements in the soil, including manganese (Mn). Our previous studies have shown that selenium (Se) ions can reduce the toxic actions of metal stress. Those, we studied the effects of Mn—treated as a stressor and Se – as a potential defense in plants. Methods Mn ions (10 mM) or/and Se (15 μM) were added into hydroponic nutrients of two wheat cultivares. The evaluation of the stress-generating and protective actions were analyzed by biochemical methods and microscopic observations in leaves and roots. Moreover the level of DNA methylation for these tissues was determined. Results Mn application caused an increase of lipid peroxidation and hydrogen peroxide content in both leaves and roots and was accompanied with a greater absorption of this element by the roots. For other elements (K, Fe, S, P), with the exception of Ca, the reduced their uptake was registered, especially in roots. For roots, Mn stimulated greater, microscopically observed, desorganization in cell structure as compared to leaves, which was accompanied by a quantitative increase in 5-methylcytosine (5-metC) in root meristem. Se application diminished the effects of Mn-stress. Conclusions These studies is the first in which indicated that global 5-metC level in roots enhancing from dividing meristematic cells to elongating cells of the axial cylinder and cortex. It was suggested that the rise in Ca level can lead to modification of root cells differentiations what may be one of the steps in defense mechanisms.