Selection of optimal reference genes for expression analysis in the entomopathogenic fungus Beauveria bassiana during development, under changing nutrient conditions, and after exposure to abiotic stresses

• Published in: Applied microbiology and biotechnology Vol. 93; no. 2; pp. 679 - 685
• Main Authors: Zhou, Yong-Hong, Zhang, Yong-Jun, Luo, Zhi-Bing, Fan, Yan-Hua, Tang, Gui-Rong, Liu, Li-Ji, Pei, Yan
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 2012; Springer Nature B.V
• Subjects: Abiotic stress; Antifungal Agents - pharmacology; Applied Genetics and Molecular Biotechnology; Beauveria - drug effects; Beauveria - genetics; Beauveria - metabolism; Beauveria bassiana; Biomedical and Life Sciences; Biotechnology; Culture Media - chemistry; DNA, Fungal - chemistry; DNA, Fungal - genetics; Fungi; Gene expression; Gene Expression Profiling - methods; Gene Expression Profiling - standards; Gene Expression Regulation, Fungal; Life Sciences; Microbial Genetics and Genomics; Microbiology; Molecular Sequence Data; Polymerase chain reaction; Proteins; Real-Time Polymerase Chain Reaction; Reference Standards; Sequence Analysis, DNA; Studies; China; Reference genes; Selection; Quantitative PCR
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-011-3561-3

The selection of suitable reference genes is crucial for accurate quantification of gene expression. To identify suitable reference genes in Beauveria bassiana , the expression of 14 candidates ( 18S , 28S , β-Tub , GAPD , γ-Act , TEF , HGPT , His3 , His2A , TBP , CypA , CypB , PP1 , and CrzA ) was measured by quantitative polymerase chain reaction at different development stages and under various nutritional and stress conditions. Expression stability, as evaluated by the geNorm and NormFinder programs, revealed that His2A / γ-Act / CrzA was the most stably expressed set of genes throughout development, while 28S / PP1 / CypA and His2A / γ-Act / CypA were the most stably expressed gene sets under a variety of nutritional and stress conditions, respectively. Overall, the most stably expressed genes under all conditions examined were PP1 , γ-Act , and CypA .