Immunological profiling and development of a sensing device for detection of IL-13 in COPD and asthma

• Published in: Bioelectrochemistry (Amsterdam, Netherlands) Vol. 143; p. 107971
• Main Authors: Choudhury, Priyanka, Biswas, Souvik, Singh, Gyanendra, Pal, Arijit, Ghosh, Nilanjana, Ojha, Atul Kumar, Das, Soumen, Dutta, Gorachand, Chaudhury, Koel
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2022; Elsevier BV
• Subjects: Asthma; Asthma - blood; Asthma - diagnosis; Asthma - immunology; Biomarkers; Biomarkers - blood; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Chronic obstructive pulmonary disease; COPD; Diagnosis; Diagnosis, Differential; Differential diagnosis; Electrochemical sensor; Female; Humans; IL13; Immunology; Interleukin 13; Interleukin 4; Interleukin 5; Interleukin 6; Interleukin-13 - blood; Interleukin-13 - immunology; Lung diseases; Male; Middle Aged; Monocyte chemoattractant protein 1; Obstructive lung disease; Patients; Pulmonary Disease, Chronic Obstructive - blood; Pulmonary Disease, Chronic Obstructive - diagnosis; Pulmonary Disease, Chronic Obstructive - immunology; Self-assembled monolayer; Self-assembled monolayers; Self-assembly; γ-Interferon; 3-MPA; Q2; MCP-1; ITO; EDC; ATR-FTIR; AFM; SPE; DPV; YKL-40; IFN-α; BSA; IFN-γ; GM-CSF; ANOVA; NHS; Self-assembled monolayer; TGF-β; Differential diagnosis; Electrochemical sensor; FENO; R2; PBS; TSLP; IL13; PLS-DA; IL-13; EIS; Asthma; SWV; CV; TNF-α; FEV1; VIP; SAM; COPD
• ISSN: 1567-5394; 1878-562X; 1878-562X
• DOI: 10.1016/j.bioelechem.2021.107971

[Display omitted] •Multiplexed analysis of 8 important immunological mediators in COPD and asthma.•Robust supervised multivariate models created using multiplex analysis data.•IL-13 exhibited maximum potential for class separation between COPD and asthma.•A reliable impedimetric IL-13 sensor is developed with 98.13% average accuracy. Chronic obstructive pulmonary disease (COPD) and asthma are the two most common obstructive lung diseases which affects millions worldwide and impose an enormous burden on global healthcare. The overlapping features shared by these two diseases often make differential diagnosis difficult to achieve, leading to misdiagnosis of these patients. Both asthma and COPD are associated with chronic inflammation of the airways which is perpetuated by the interplay between immunological mediators. The crucial role played by these mediators make them attractive targets for disease diagnosis. The present study investigates the immunological mediator profile in these patients as compared with controls. Further, a potential biomarker for the development of a sensing platform is identified. Multiplexed analysis of 8 commonly studied immunological markers (IL-4, IL-5, IL-6, IL-13, TGF-β, IFN-γ, MCP-1 and NGAL) in serum showed distinct dysregulation pattern, with IL-13 showing the highest potential for differential diagnosis. An impedimetric self-assembled monolayer (SAM) based sensor for detecting IL-13 is developed to distinguish between asthma and COPD. The device shows reliable output with high accuracy and sensitivity towards the detection of IL-13.