Establishment of efficient and rapid regeneration system for some diploid wild species of Arachis

• Published in: Plant cell, tissue and organ culture Vol. 101; no. 3; pp. 303 - 309
• Main Authors: Srinivasan, Tantravahi, Kumar, Koppolu Raja Rajesh, Kirti, Pulugurtha Bharadwaja
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.06.2010; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: acclimation; Acetic acid; adventitious shoots; Arachis; Arachis hypogaea; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Buds; cotyledons; culture media; Cytokinins; diploidy; Elongation; Explants; Fundamental and applied biological sciences. Psychology; in vitro regeneration; Life Sciences; mortality; Naphthalene; naphthaleneacetic acid; Nodes; Original Paper; Peanuts; plant genetic resources; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Regeneration; rooting; Seedlings; shoot meristems; Shoots; Species; species differences; Stenosperma; Tissue culture; Transgenic plants; wild plants; wild relatives; TDZ–thidiazuron; Wild; Shoot regeneration; KIN–6; species; BA-6; Benzyl amino purine; Cotyledonary node explants; Fufuryl amino purine; Urine; Benzyl amino urine; Vegetals; Cotyledon; Node; Explant; Wild Arachis species; Regeneration; Diploidy; Wild plant; Purine; KIN-6; Shoot; TDZ-thidiazuron
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-010-9689-5

Though peanut tissue culture has advanced to a considerable extent using a number of explants with the subsequent production of transgenic plants, wild Arachis species appeared to be recalcitrant to using similar explants. In this study, the use of cotyledonary nodes as explants prepared from 7-day old seedlings resulted in the development of a simple and rapid regeneration protocol for five diploid wild species including A. diogoi, A. stenosperma, A. duranensis, A. cardenasii and A. correntina belonging to the genus Arachis for producing multiple shoots. Shoot bud initiation was observed 10 to 15 days after culture initiation. Responding cotyledonary nodes with shoot buds were subcultured to lower levels of cytokinin and finally to MS basal medium for further shoot development and elongation. Production of multiple shoots was observed in all the five diploid species with a maximum of 9 to 16 shoots were obtained per explant in the primary cultures. The number of shoot buds increased significantly with repeated explant subculturing with recovery up to 45 shoots from responding explants. These shoots were rooted efficiently on MS medium supplemented with 1 mg l⁻¹ naphthalene acetic acid and the time taken from explanting to the transfer of shoots to potting mixture was about 12 weeks. All rooted shoots were successfully established in soil in glass house and further transferred to field. These plants survived to maturity and set seed.