Aquaporin expression in response to different water stress intensities and recovery in Richter-110 (Vitis sp.): relationship with ecophysiological status

• Published in: Planta Vol. 226; no. 3; pp. 671 - 681
• Main Authors: Galmés, Jeroni, Pou, Alícia, Alsina, Maria Mar, Tomàs, Magdalena, Medrano, Hipólito, Flexas, Jaume
• Format: Journal Article
• Language: English
• Published: Berlin Berlin/Heidelberg : Springer-Verlag 01.08.2007; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Abscisic acid; Acclimation; Acclimatization; Agronomy. Soil science and plant productions; Aquaporins; Aquaporins - genetics; Biological and medical sciences; Conductance; Dehydration; Desiccation; Drought; Drought resistance; Economic plant physiology; Ecosystem; Flowers & plants; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Profiling; Homeostasis; Leaves; Photosynthesis; Plant cells; Plant Leaves - metabolism; Plant roots; Plant Roots - metabolism; Plants; Recovery; Richter-110; Stomata; Stomatal conductance; Stress; Vitis; Vitis - genetics; Vitis - physiology; Water availability; Water potential; Water relations, transpiration, stomata; Water stress; Xylem; Xylem - metabolism; Richter-110; Vitidaceae; Dicotyledones; Vitis; Aquaporins; Angiospermae; Spermatophyta; Drought; Vitis, Water stress; Water stress; Recovery; Aquaporin
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-007-0515-1

Aquaporins seem essential for the regulation of plant water status and expenses. Richter-110 is a Vitis hybrid (Vitis berlandieri x rupestris) reputed to be strongly drought-tolerant. Three irrigation treatments were established in Richter-110 plants growing outdoors defined by the resulting maximum stomatal conductance (g s), and ensuring water stress situations not severe enough as to stop photosynthesis and growth: well-watered plants (g s about 250 mmol H₂O m-² s-¹), moderate water stress (g s about 150 mmol H₂O m-² s-¹) and severe water stress (g s about 50 mmol H₂O m-² s-¹). Plants under water stress were kept at constant water availability for 7 days to check for possible acclimation. Finally, plants were re-watered, and allowed to recover, for 3 days. Stomatal conductance, leaf water potential, xylem abscisic acid (ABA) content and root and stem hydraulic conductivity were determined. The relative amounts of expression of mRNA encoding seven putative aquaporins were determined in roots and leaves by RT-PCR. The decrease in stomatal conductance with moderate and severe water stress was associated with increasing ABA contents, but not with the leaf water potential and hydraulic conductivities, which remained unchanged during the entire experiment. Aquaporin gene expression varied depending on which aquaporin, water stress level and the plant organ. We suggest that aquaporin expression was responsive to water stress as part of the homeostasis, which resulted in constant leaf water potential and hydraulic conductivity.