Ultrastructure analysis of cadmium-tolerant and -sensitive cell lines of cucumber (Cucumis sativus L.)

• Published in: Plant cell, tissue and organ culture Vol. 99; no. 2; pp. 227 - 232
• Main Authors: Gzyl, Jarosław, Przymusiński, Roman, Gwóźdź, Edward A
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.11.2009; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Cadmium; Cadmium chloride; Cell culture; Cell lines; cell nucleus; cell suspension culture; Cellular structure; cucumbers; Cucumis sativus; culture media; Cytoplasm; Disintegration; Endoplasmic reticulum; Exposure; Fundamental and applied biological sciences. Psychology; genetic variation; Globules; Life Sciences; metal tolerance; Mitochondria; Nucleoli; organelles; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Research Note; Suspension culture; Transmission electron microscopy; Ultrastructure; Vacuoles; Tolerance; Cadmium; Cell suspension; Ultrastructure; Cell culture; Vegetals; Cucurbitaceae; Cucumis sativus; Suspension culture; Cell line; Sensitivity resistance; Dicotyledones; Angiospermae; Spermatophyta; Cucumber
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-009-9583-1

Previously, a stable cell suspension culture of cucumber tolerant to cadmium (Cd) was established (Gzyl and Gwóźdź, Plant Cell Tissue Organ Cult 80:59-67, 2005). In this study, ultrastructures of Cd-tolerant and -sensitive cells were analyzed by transmission electron microscopy (TEM). Ultrastructural differences between cell lines exposed to 100 μM CdCl₂ were observed both at cellular and organelle levels. Tolerant cells exposed to Cd exhibited well-preserved cellular structures in comparison with sensitive cells. Increased numbers of osmiophilic globules in the cytoplasm and nucleolus-associated bodies as well as electron dense material in vacuoles were observed in cadmium tolerant cells. In contrast, ultrastructure of sensitive cells following exposure to Cd exhibited distinct disturbances including vacuolation, disintegration of cytoplasm, and structural changes in both mitochondria and endoplasmic reticulum. TEM observations confirmed the adaptation of tolerant cells to Cd.