Expression and clinical role of small glutamine-rich tetratricopeptide repeat (TPR)-containing protein alpha (SGTA) as a novel cell cycle protein in NSCLC

• Published in: Journal of cancer research and clinical oncology Vol. 139; no. 9; pp. 1539 - 1549
• Main Authors: Xue, Qun, Lv, Liting, Wan, Chunhua, Chen, Buyou, Li, Mei, Ni, Tingting, Liu, Yifei, Liu, Yanhua, Cong, Xia, Zhou, Yiqun, Ni, Runzhou, Mao, Guoxin
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2013; Springer; Springer Nature B.V
• Subjects: Adenocarcinoma - metabolism; Adenocarcinoma - mortality; Adult; Aged; Antineoplastic agents; Apoptosis; Biological and medical sciences; Biomarkers; Biomarkers, Tumor - metabolism; Blotting, Western; Cancer Research; Carcinoma, Non-Small-Cell Lung - metabolism; Carcinoma, Non-Small-Cell Lung - mortality; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - mortality; Carrier Proteins - metabolism; Cell Cycle; Cell Proliferation; Cohort Studies; Female; Follow-Up Studies; Hematology; Humans; Immunoenzyme Techniques; Internal Medicine; Lung - metabolism; Lung cancer; Lung Neoplasms - metabolism; Lung Neoplasms - mortality; Male; Medical prognosis; Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Multiple tumors. Solid tumors. Tumors in childhood (general aspects); Neoplasm Grading; Neoplasm Staging; Oncology; Original Paper; Pharmacology. Drug treatments; Pneumology; Prognosis; Proteins; Survival Rate; Tumor Cells, Cultured; Tumors; Tumors of the respiratory system and mediastinum; Non-small-cell lung cancer; Cell division; Prognosis; Proliferation; SGTA; Cell proliferation; Lung disease; Respiratory disease; Lung cancer; Malignant tumor; non-small cell lung carcinoma; Gene expression; Protein; Aminoacid; Cell cycle; Bronchus disease; Cancer; Glutamine
• ISSN: 0171-5216; 1432-1335
• DOI: 10.1007/s00432-013-1474-5

Purpose A small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a 35 kDa protein involved in a number of biological processes. However, the role of SGTA in non-small-cell lung cancer (NSCLC) tumorigenesis has never been elucidated. The purpose of this study was to determine whether SGTA could serve as a biomarker for stratification and prediction of prognosis in NSCLC. Methods Small glutamine-rich tetratricopeptide repeat-containing protein alpha expression was evaluated by Western blot in 8 paired fresh lung cancer tissues and immunohistochemistry on 83 paraffin-embedded sections. The effect of SGTA was assessed by RNA interference in A549 cells. Serum starvation and refeeding, flow cytometry, CCK-8, and tunnel assays were performed. Results Small glutamine-rich tetratricopeptide repeat-containing protein alpha was highly expressed in NSCLC and significantly correlated with NSCLC histological differentiation, clinical stage, and Ki-67. Multivariate analysis indicated that SGTA was an independent prognostic factor for NSCLC patients’ survival. The present investigation demonstrated that suppression of SGTA expression resulted in a significant decline of proliferation in A549 cells. Besides, SGTA could abolish the toxicity of cisplatin in A549 cells. Conclusions These findings suggested that SGTA might play an important role in promoting the tumorigenesis of NSCLC, and thus be a promising therapeutic target to prevent NSCLC progression.