Trends and progress in the characterization of stationary phases for reversed-phase liquid chromatography

• Published in: TrAC, Trends in analytical chemistry (Regular ed.) Vol. 20; no. 10; pp. 563 - 583
• Main Author: Claessens, H.A
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.2001; Elsevier Science
• Subjects: Analytical chemistry; Batch-to-batch repeatabilities; Chemical stability; Chemistry; Chromatographic methods and physical methods associated with chromatography; Column lifespan; Column testing and selection; Eluent properties; Exact sciences and technology; Other chromatographic methods; Selectivity; Testing parameters; Column testing and selection; Batch-to-batch repeatabilities; Chemical stability; Selectivity; Testing parameters; Column lifespan; Eluent properties; Separation method; Reversed phase chromatography; Analysis method; Liquid chromatography; Property structure relationship; Review; Bonded stationary phase
• ISSN: 0165-9936; 1879-3142
• DOI: 10.1016/S0165-9936(01)00098-X

The state-of-the-art and progress in the characterization of reversed-phase liquid chromatographic (RPLC) columns are discussed. Existing column tests are compared with tests under development. The validity of several test parameters, e.g. hydrophobicity and silanol activity, is also discussed. Solvent and eluent properties are treated with respect to retention and selectivity. Developments to improve the chemical stability of RPLC phases and thus column lifetime are reviewed. It is shown that substantial progress has been made in the manufacturing of RPLC columns, enabling their use over a wide variety of experimental conditions. In addition, general measures to significantly extend their lifespan are discussed. The substantial improvements in batch-to-batch and column-to-column repeatabilities achieved during the last two decades are also reviewed.