Isolation and identification of Amycolatopsis sp. strain 1119 with potential to improve cucumber fruit yield and induce plant defense responses in commercial greenhouse
Background and aims The application of chemical fungicides is the first strategy to control plant fungal diseases. This approach is highly polluting for the environment and affects human health. The artificial introduction of beneficial rhizobacteria into the soil can be an economical and practical...
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Published in | Plant and soil Vol. 468; no. 1-2; pp. 125 - 145 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer International Publishing
01.11.2021
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Background and aims
The application of chemical fungicides is the first strategy to control plant fungal diseases. This approach is highly polluting for the environment and affects human health. The artificial introduction of beneficial rhizobacteria into the soil can be an economical and practical way to control phytopathogenic fungi in commercial greenhouses. Here, we recount the travel of a rare Actinomycete (
Amycolatopsis
strain 1119) from a maize field to a commercial cucumber greenhouse.
Methods and results
Culturable bacteria from rhizosphere and bulk soils of dicot and monocot crops were isolated and screened. About 20% of the representative colonies showed Actinobacteria appearance. 106 Actinobacteria that had antagonistic activity against
Phytophthora capsici
and were able to produce IAA-like molecules were selected for further analysis. Two
Streptomyces
strains (432 and 615) and 2
Amycolatopsis
strains (3513 and 1119) that showed a positive effect on plant growth in greenhouse conditions were selected to evaluate the biocontrol potential. Strains 432, 3513, 615 and 1119 controlled incidence of the damping-off by 65%, 42%, 83% and 100% respectively. Application of strain 1119 under commercial greenhouse conditions resulted in an increase in fruit yield (20%) and a decrease in fruit nitrate content (70%). Increased antioxidant enzymes activity and increased
LOX
and
APX
transcription and also, increased expression of two genes
PR1-1a
and
GLU
(SAR genes) showed that strain 1119 could induce both ISR and SAR in cucumber without pathogen exposure.
Conclusion
Our results demonstrate that the
Amycolatopsis
strain 1119 has a great potential to be used as an active principle for bio-inoculant development because of the ability to improve cucumber fruit yield and induce plant defense responses in a commercial greenhouse. |
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ISSN: | 0032-079X 1573-5036 |
DOI: | 10.1007/s11104-021-05097-3 |