OCCURRENCE OF GLUTAMINE-2-OXOACID TRANSAMINASE ACTIVITY IN THE BLUE-GREEN ALGA ANABAENA CYLINDRICA
Aspartate and glutamate were formed from glutamine and oxaloacetate with crude enzyme extracts obtained by ammonium sulfate precipitation (35-65% saturation) from the blue-green alga Anabaena cylindrica. In this reaction the 2-amino group of glutamine was transferred to oxaloacetate in the absence o...
Saved in:
Published in | Journal of general and applied microbiology Vol. 31; no. 2; pp. 171 - 176 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Tokyo
Applied Microbiology, Molecular and Cellular Biosciences Research Foundation
1985
Microbiology Research Foundation |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Aspartate and glutamate were formed from glutamine and oxaloacetate with crude enzyme extracts obtained by ammonium sulfate precipitation (35-65% saturation) from the blue-green alga Anabaena cylindrica. In this reaction the 2-amino group of glutamine was transferred to oxaloacetate in the absence of a reluctant. Azaserine, an inhibitor of glutamine -amide transfer, inhibited glutamate formation from glutamine but did not inhibit aspartate formation. Thus, a transamination from glutamine directly to oxaloacetate was indicated. This transamination reaction may be accomplished with glutamine-2-oxoacid transaminase which is found in animal tissues and in yeast. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-1260 1349-8037 |
DOI: | 10.2323/jgam.31.171 |