OCCURRENCE OF GLUTAMINE-2-OXOACID TRANSAMINASE ACTIVITY IN THE BLUE-GREEN ALGA ANABAENA CYLINDRICA

Aspartate and glutamate were formed from glutamine and oxaloacetate with crude enzyme extracts obtained by ammonium sulfate precipitation (35-65% saturation) from the blue-green alga Anabaena cylindrica. In this reaction the 2-amino group of glutamine was transferred to oxaloacetate in the absence o...

Full description

Saved in:
Bibliographic Details
Published inJournal of general and applied microbiology Vol. 31; no. 2; pp. 171 - 176
Main Authors OHMORI, MASAYUKI, SAINO, TOSHIRO, OHMORI, KAZUKO
Format Journal Article
LanguageEnglish
Published Tokyo Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 1985
Microbiology Research Foundation
Subjects
Anabaena cylindrica
Bacteriology
Biological and medical sciences
Freshwater
Fundamental and applied biological sciences. Psychology
Metabolism. Enzymes
Microbiology
Characterization
Glutamine―oxo-acid aminotransferase
Bacteria
Online AccessGet full text

Cover

More Information
Summary:Aspartate and glutamate were formed from glutamine and oxaloacetate with crude enzyme extracts obtained by ammonium sulfate precipitation (35-65% saturation) from the blue-green alga Anabaena cylindrica. In this reaction the 2-amino group of glutamine was transferred to oxaloacetate in the absence of a reluctant. Azaserine, an inhibitor of glutamine -amide transfer, inhibited glutamate formation from glutamine but did not inhibit aspartate formation. Thus, a transamination from glutamine directly to oxaloacetate was indicated. This transamination reaction may be accomplished with glutamine-2-oxoacid transaminase which is found in animal tissues and in yeast.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-1260
1349-8037
DOI:10.2323/jgam.31.171