Multiplex bioimaging of piRNA molecular pathway-regulated theragnostic effects in a single breast cancer cell using a piRNA molecular beacon

• Published in: Biomaterials Vol. 101; pp. 143 - 155
• Main Authors: Lee, Youn Jung, Moon, Sung Ung, Park, Min Geun, Jung, Woon Yong, Park, Yong Keun, Song, Sung Kyu, Ryu, Je Gyu, Lee, Yong Seung, Heo, Hye Jung, Gu, Ha Na, Cho, Su Jeong, Ali, Bahy A, Al-Khedhairy, Abdulaziz A, Lee, Ilkyun, Kim, Soonhag
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.09.2016
• Subjects: Acyltransferases - genetics; Advanced Basic Science; alpha 1-Antitrypsin - genetics; Animals; Base Sequence; Breast - diagnostic imaging; Breast - metabolism; Breast - pathology; Breast Neoplasms - diagnostic imaging; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Cancer theragnosis; Dentistry; Female; Gene Expression Regulation, Neoplastic; HEK293 Cells; Humans; Male; MCF-7 Cells; Mice, Inbred BALB C; Mice, Nude; Molecular beacon; Molecular pathway; Multiplex bioimaging; Oligonucleotide Probes - analysis; Oligonucleotide Probes - genetics; Optical Imaging; piRNA-36026; RNA, Small Interfering - analysis; RNA, Small Interfering - genetics; Multiplex bioimaging; Molecular beacon; Cancer theragnosis; piRNA-36026; Molecular pathway
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2016.05.052

Abstract Recently, PIWI-interacting small non-coding RNAs (piRNAs) have emerged as novel cancer biomarkers candidate because of their high expression level in various cancer types and role in the control of tumor suppressor genes. In this study, a novel breast cancer theragnostics probe based on a single system targeting the piRNA-36026 (piR-36026) molecular pathway was developed using a piR-36026 molecular beacon (MB). The piR-36026 MB successfully visualized endogenous piR-36026 biogenesis, which is highly expressed in MCF7 cells (a human breast cancer cell line), and simultaneously inhibited piR-36026-mediated cancer progression in vitro and in vivo . We discovered two tumor suppressor proteins, SERPINA1 and LRAT, that were directly regulated as endogenous piR-36026 target genes in MCF7 cells. Furthermore, multiplex bioimaging of a single MCF7 cell following treatment with piR-36026 MB clearly visualized the direct molecular interaction of piRNA-36026 with SERPINA1 or LRAT and subsequent molecular therapeutic responses including caspase-3 and PI in the nucleus.