Immobilization of enzymes with polyaziridines

• Published in: Journal of biotechnology Vol. 13; no. 4; pp. 305 - 314
• Main Authors: Wood, Louis L., Cobbs, Carrington S., Lantz, Leon, Peng, Lin, Calton, Gary J.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.03.1990; Amsterdam Elsevier; New York, NY
• Subjects: Biological and medical sciences; Biotechnology; Enzyme immobilization; Enzymes, Immobilized; Fundamental and applied biological sciences. Psychology; Immobilization of enzymes and other molecules; Immobilization techniques; Methods. Procedures. Technologies; Polyaziridine; Polyethyleneimine - chemical synthesis; Polyethylenes - chemical synthesis; Polyaziridine; Enzyme immobilization; Performance evaluation; Temperature; Enzymatic activity; Support; Enzyme; Immobilization; Covalent bond; Environmental factor
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/0168-1656(90)90078-P

A novel method of enzyme immobilization using a low molecular weight prepolymer of tri-functional aziridines which can immobilize enzymes both by covalent attachment and entrapment within a gel matrix is described. The enzymes are immobilized on a solid support and exhibit an excellent retention of enzymatic activity. The immobilization procedure is essentially a single step process which can be easily performed at room temperature or 4°C in either aqueous solution or in an inert organic solvent. The polyaziridines used in the immobilization are nontoxic, available in bulk at low cost and completely miscible with water and many organic solvents, thus providing one of the most satisfactory methods of immobilization available.