Enzymatically assisted isolation of high-quality cellulose nanoparticles from water hyacinth stems

• Published in: Carbohydrate polymers Vol. 220; pp. 110 - 117
• Main Authors: Juárez-Luna, Gregorio N., Favela-Torres, Ernesto, Quevedo, Iván R., Batina, Nikola
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.09.2019
• Subjects: AFM; Cellulase complex; Cellulose nanocrystals; Cellulose nanoparticles; Enzymatic hydrolysis; Water hyacinth stems; XRD; CNF-BP; Cel-A; Cel-WH; WHS; Cellulase complex; CNP; Enzymatic hydrolysis; AFM; Water hyacinth stems; XRD; CNP-BP; FTIR; Cellulose nanocrystals; CNC; CNP-AV; Cellulose nanoparticles; CNF; SEM; NTA; CNP-WH
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2019.05.058

[Display omitted] •The high quality cellulose nanoparticles, were obtained from water hyacinth stems.•The obtained nanoparticles possesses high crystallinity, defined form and size.•The enzymatic hydrolysis is environmentally friendly process. High quality cellulose nanoparticles (CNP) were isolated from water hyacinth stem cellulose (Cel-WH) extracted via successive thermochemical and alkaline-peroxide treatments, and further enzymatically hydrolysed using the commercial cellulase complex, NS22086, at 50ºC. The maximum CNP concentration was reached after 120 min of enzymatic hydrolysis, with a hydrodynamic diameter in the order of 200−250 nm and an increase of 5% in crystallinity as compared with Cel-WH. The obtained rod-shaped cellulose nanocrystals, as revealed by atomic force microscopy (AFM), exhibited a nominal diameter of 15.6−29.4 nm, a length of 56−184.8 nm, and a height of 2.85−6.43 nm, indicating a low tendency to form aggregates. In the present study, it was found that water hyacinth stems are a valuable source for the isolation of high-quality CNP using an environmentally friendly procedure, with potential applications in nanomedicine and nanopharmacology.