IGFBP-3 Inhibits the Proliferation of Neural Progenitor Cells

• Published in: Neurochemical research Vol. 36; no. 3; pp. 406 - 411
• Main Authors: Kalluri, Haviryaji S. G., Dempsey, Robert J.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.03.2011; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cell Proliferation - drug effects; Cells, Cultured; Insulin-Like Growth Factor Binding Protein 3 - pharmacology; Insulin-Like Growth Factor I - pharmacology; Neural Stem Cells - cytology; Neural Stem Cells - drug effects; Neural Stem Cells - physiology; Neurochemistry; Neurology; Neurosciences; Original Paper; Rats; Neural stem cells; Retinoblastoma; Neurogenesis; Cell cycle
• ISSN: 0364-3190; 1573-6903
• DOI: 10.1007/s11064-010-0349-2

Insulin like growth factor-1 (IGF-1) plays an important role in the proliferation and differentiation of neural progenitor cells. The effects of IGF-1 can be regulated by insulin like growth factor binding protein-3 (IGFBP-3) which can either inhibit or stimulate the proliferation of cells depending on the expression of proteases that can release IGF-1 from IGF1-IGFBP3 complex. Although IGF-1 is essential for the development of brain, both IGFBP-3 and IGF-1 are elevated in the brains of children younger than 6 months of age. Likewise, IGFBP-3 is also upregulated following cerebral ischemia and hypoxia. However, the role of IGFBP-3 in neurogenesis is not clear. Using an in vitro culture system of rat neural progenitor cells, we demonstrate that IGFBP-3 specifically regulates the IGF-1 mediated neural progenitor cell proliferation via down regulation of phopho-Akt, and cyclin D1. In addition, IGFBP-3 also decreased the content of nestin in the neural progenitor cells indicating its potential role in neurogenesis.