Molecular characterization of Moroccan fig germplasm using intersimple sequence repeat and simple sequence repeat markers to establish a reference collection

• Published in: HortScience Vol. 40; no. 1; pp. 29 - 32
• Main Authors: Khadari, B, Oukabli, A, Ater, M, Mamouni, A, Roger, J.P, Kjellberg, F
• Format: Journal Article
• Language: English
• Published: Alexandria, VA American Society for Horticultural Science 01.02.2005
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; cultivar identification; Ficus carica; figs; Fundamental and applied biological sciences. Psychology; genetic markers; genotype; geographical distribution; germplasm; germplasm conservation; Life Sciences; loci; microsatellite repeats; plant genetic resources; Morocco; Horticulture; Repeated sequence; Reference; genetic relationships; Genotype; molecular characterization; Moraceae; ISSR; Ficus carica; intersimple sequence repeats; reference genotype; Characterization; SSR; Dicotyledones; Microsatellite DNA; Angiospermae; Ficus carica L; Genetics; Spermatophyta; simple sequence repeats; INTERSIMPLE SEQUENCE; MOLECULAR CHARACTERIZATION; MORPHOLOGICAL TRAIT; FIGUIER; BIOLOGIE MOLECULAIRE; SIMPLE SEQUENCE REPEATS; GENETIC RELATIONSHIP; MOLECULAR MARKER; REFERENCE GENOTYPE
• ISSN: 0018-5345; 2327-9834
• DOI: 10.21273/HORTSCI.40.1.29

A study was conducted to identify genotypes present in a Moroccan fig germplasm collection and provide the first database for a reference collection in northern Morocco. In total, 75 fig samples were analyzed using 8 intersimple sequence repeat primers and 6 simple sequence repeat loci. From these samples, we identified 72 fig genotypes. In genetically heterogeneous cultivars, genotypes under the same denomination were distinguished by both molecular markers and pomological traits. Molecular analysis was used to classify the germplasm into 46 well-defined cultivars and 6 caprifig trees. The remaining genotypes were not clearly identified due to three cases of mislabeling and four cases of homonymy. No evidence was found for the occurrence of geographically widespread genotypes.