Genotyping of CCR5 Gene, CCR2b and SDF1 Variants Related to HIV-1 Infection in Gabonese Subjects

• Published in: Intervirology Vol. 58; no. 1; pp. 22 - 26
• Main Authors: Mombo, Landry Erik, Bisseye, Cyrille, Mickala, Patrick, Ossari, Simon, Makuwa, Maria
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.01.2015
• Subjects: Chemokine CXCL12 - genetics; Denaturing Gradient Gel Electrophoresis; Environment; Gabon - epidemiology; Genetic Variation; Genotype; HIV Infections - epidemiology; HIV Infections - genetics; HIV Seroprevalence; HIV-1 - immunology; Human immunodeficiency virus; Human immunodeficiency virus 1; Humans; Open Reading Frames; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Prevalence; Receptors, CCR2 - genetics; Receptors, CCR5 - genetics; Short Communication; Gabon; HIV-1; CCR5 gene; Gabon; SDF1; CCR2b
• ISSN: 0300-5526; 1423-0100; 1423-0100
• DOI: 10.1159/000369016

Objective: Given the magnitude of the HIV epidemic infection, many viral and human factors were analyzed, and the most decisive was the variant CCR5-Δ32. The presence of a low HIV prevalence (1.8%) in Gabon in the 1990s, compared to neighboring countries, represents a paradox that led us to search for viral and human genetic variants in this country. In this study, only variants of coreceptors and chemokines were investigated. Methods: Variants of the coding region of the CCR5 gene were analyzed by denaturing gradient gel electrophoresis, and then variants of SDF1 and CCR2b were determined by polymerase chain reaction-restriction fragment length polymorphism. Results: Four rare variants of the CCR5 coreceptor were found, while CCR5-Δ32 and CCR5m303 variants were not found. No association with CCR2b-V64I (17%) and SDF1-3′A (2%) variants was determined in relation to HIV-1 infection in Gabonese patients. Conclusion: The paradox of HIV seroprevalence in Gabon, which ended in the 2000s, was not caused by human genetic variants but rather by environmental factors.