Influence of Silver Nitrate on Somatic Embryogenesis Induction in Arabica Coffee (Coffea arabica L.)

Abstract The influence of silver nitrate (AgNO3), benzyladenine (BAP), and indole-3-acetic acid (IAA) on low frequency somatic embryogenesis (LFSE) induction in Caturra and Catuaí arabica coffee was evaluated. For the Caturra cultivar, the production of somatic embryos was significantly increased by...

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Published inBrazilian Archives of Biology and Technology Vol. 62
Main Authors Rojas-Lorz, Laura, Arrieta-Espinoza, Griselda, Valdez-Melara, Marta, Pereira, Luiz Filipe Protasio, Gatica-Arias, Andrés
Format Journal Article
LanguageEnglish
Published Instituto de Tecnologia do Paraná (Tecpar) 2019
Subjects
coffee
plant growth regulators
silver nitrate
somatic embryos
tissue culture
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Summary:Abstract The influence of silver nitrate (AgNO3), benzyladenine (BAP), and indole-3-acetic acid (IAA) on low frequency somatic embryogenesis (LFSE) induction in Caturra and Catuaí arabica coffee was evaluated. For the Caturra cultivar, the production of somatic embryos was significantly increased by adding AgNO3 to the semisolid culture medium. The highest average number of somatic embryos for this cultivar was obtained using 6.6 μM BAP, 2.85 μM IAA, and 40 μM AgNO3. In contrast, for the Catuaí cultivar, the highest average number of somatic embryos was obtained using semisolid medium supplemented with 8.8 μM BAP, and 2.85 μM IAA. Using these protocols, somatic embryos were directly induced using leaf sections of in vitro plants of both coffee cultivars within 8 weeks. The somatic embryos developed into rooted plants with a 100% survival rate upon transfer to the greenhouse.
ISSN:1516-8913
1678-4324
1678-4324
DOI:10.1590/1678-4324-2019180228