Production of nano bacterial cellulose from beverage industrial waste of citrus peel and pomace using Komagataeibacter xylinus

• Published in: Carbohydrate polymers Vol. 151; pp. 1068 - 1072
• Main Authors: Fan, Xin, Gao, Yue, He, Wanying, Hu, Hao, Tian, Ming, Wang, Kexing, Pan, Siyi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 20.10.2016
• Subjects: Acetobacteraceae - metabolism; Bacterial cellulose; Beverages; Biotechnology; Cellulose - biosynthesis; Cellulose - chemistry; Citrus - chemistry; Citrus peel and pomace enzymolysis; Color; Culture Media - chemistry; Fermentation; Fruit - chemistry; Industrial Waste; Komagataeibacter xylinus; Nanotechnology; Glucose (PubChem CID: 5793); Phenol (PubChem CID: 996); Komagataeibacter xylinus; Sulfuric acid (PubChem CID: 1118); Disodium hydrogen phosphate (PubChem CID: 24203); Citric acid (PubChem CID: 311); Potassium bromide (PubChem CID: 253877); Sodium hydroxide (PubChem CID: 14798); 3,5-dinitrosalicylic acid (PubChem CID: 11873); Citrus peel and pomace enzymolysis; Yeast extract (PubChem CID: 24973165); Bacterial cellulose; Ethanol (PubChem CID: 702)
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2016.06.062

•Application of citrus peel and pomace enzymolysis (CPPE) was firstly explored as medium.•CPPE was used as medium to produce bacterial cellulose(BC).•Komagataeibacter xylinus was used for BC production.•The yield of BC from CPPE medium and Hestrin−Schramm(HS) medium were 5.7±0.7g/L and 3.9±0.6g/L,respectively.•The two varieties of BC showed no significant differences in relation to colour parameter, crystallinity index, chemical group, microstructure and melting point. Bacterial cellulose (BC) is a high-purity and robust cellulose that is utilised in medicine, consumer goods, and industrial practices. The present study aimed to investigate the suitability of beverage industrial waste for the production of BC by Komagataeibacter xylinus CICC No. 10529 and to study the structural properties of BC films in both citrus peel and pomace enzymolysis (CPPE) and Hestrin-Schramm (HS, Hestrin & Schramm, 1954) media. Under similar experimental conditions, the yield of BC from CPPE medium was 5.7±0.7g/L, which was higher than from HS medium (3.9±0.6g/L). To evaluate the structure of BC, fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), X-ray diffraction (XRD), differential scanning calorimetry (DSC), and colour evaluation using a chroma meter were utilised. The average diameters of BC, obtained from CPPE and HS mediums, were 50nm and 60nm, respectively. The crystallinity index of BC from the CPPE medium was approximately 63%, which was lower than BC produced from the HS medium (65%). The two varieties of BC showed no significant differences in relation to their colour parameters. Therefore, BC production from CPPE medium had similar properties to BC from HS medium, but it is more environmentally friendly and cheaper to produce.