Multiplex Amplified Nominal Tandem-Repeat Analysis (MANTRA), a Rapid Method for Genotyping Mycobacterium tuberculosis by Use of Multiplex PCR and a Microfluidic Laboratory Chip

A variable-number tandem-repeat genotyping method for Mycobacterium tuberculosis was converted to run in a multiplex PCR format on a 12-well microfluidic laboratory chip. Epidemiologically and genotypically distinct isolate clusters of M. tuberculosis were identified. This rapid genotyping method ha...

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Bibliographic Details
Published inJournal of Clinical Microbiology Vol. 48; no. 10; pp. 3758 - 3761
Main Authors Merritt, Adam J, Keehner, Terillee, O'Reilly, Lyn C, McInnes, Russell L, Inglis, Timothy J.J
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.10.2010
American Society for Microbiology (ASM)
Subjects
Bacterial Typing Techniques - methods
DNA Fingerprinting - methods
Humans
Microfluidic Analytical Techniques - methods
Minisatellite Repeats
Molecular Epidemiology - methods
Mycobacteriology and Aerobic Actinomycetes
Mycobacterium tuberculosis - classification
Mycobacterium tuberculosis - genetics
Polymerase Chain Reaction - methods
Tuberculosis - diagnosis
Tuberculosis - microbiology
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Summary:A variable-number tandem-repeat genotyping method for Mycobacterium tuberculosis was converted to run in a multiplex PCR format on a 12-well microfluidic laboratory chip. Epidemiologically and genotypically distinct isolate clusters of M. tuberculosis were identified. This rapid genotyping method has potential application in smaller clinical laboratories and public health field investigations.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.00471-10