Arbuscular mycorrhiza improves acclimatization and increases lignan content of micropropagated mayapple ( Podophyllum peltatum L.)

• Published in: Plant science (Limerick) Vol. 166; no. 1; pp. 23 - 29
• Main Authors: Moraes, Rita M., Andrade, Zita De, Bedir, Ebru, Dayan, Franck E., Lata, Hemant, Khan, Ikhlas, Pereira, Ana M.S.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 2004; Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; Antineoplastic drugs. Cytokines; Arbuscular mycorrhiza; Arbuscular mycorrhiza (AM); Biological and medical sciences; Biotechnology; Economic plant physiology; Entrophospora; Entrophospora colombiana; Fundamental and applied biological sciences. Psychology; Gigaspora; Gigaspora ramisporophora; Glomus mosseae; Health. Pharmaceutical industry; Industrial applications and implications. Economical aspects; Lignan; Micropropagation; Parasitism and symbiosis; Plant physiology and development; Podophyllotoxin; Podophyllum; Podophyllum peltatum; Production of active biomolecules; Scutellospora; Scutellospora fulgida; Symbiosis; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); USA, Mississippi; Arbuscular mycorrhiza (AM); Lignan; Micropropagation; Podophyllotoxin; Podophyllum; Antineoplastic agent; Symbiosis; Drug; Gigaspora; Phycomycetes; Glomus mosseae; PODOPHYLLUM; Survival; Medicinal plant; Fungi; Improvement; Endomycorrhiza; Mycorrhiza; Production; Chemical concentration; Vigor; Thallophyta; Adaptation
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2003.07.003

In vitro propagated plants are delicate and lacking vigor to survive the acclimatization shock with great losses observed frequently. Comparison of potting substrates showed that survival of Podophyllum peltatum plantlets was higher (97%) in a non-sterile soil (NS)–sand (2:1 v/v) substrate than in Miracle–Gro potting mix ®-sand (2:1 v/v) with or without inocula of arbuscular mycorrhiza (AM). The non-sterile soil substrate was collected from areas of P. peltatum wild-grown colonies located on the University of Mississippi Campus. Within the group of plantlets grown in the Miracle–Gro potting mix ®-sand substrate, the survival rate was significantly higher in plantlets inoculated with AM fungi. Plantlets inoculated with Entrophospora colombiana had a superior survival rate (73%) than plantlets inoculated with Glomus mosseae, Gigaspora ramisporophora or Scutellospora fulgida (57%). Ex vitro G. ramisporophora inoculated plants yielded more podophyllotoxin and related lignans than the control, non-inoculated plants.