Sol–gel horseradish peroxidase biosensor for hydrogen peroxide detection by chemiluminescence

• Published in: Analytica chimica acta Vol. 363; no. 2; pp. 221 - 227
• Main Authors: Navas Dı́az, A, Ramos Peinado, M.C, Torijas Minguez, M.C
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.05.1998; Elsevier
• Subjects: Biological and medical sciences; Biosensors; Biotechnology; Chemiluminescence; Fundamental and applied biological sciences. Psychology; Horseradish peroxidase; Hydrogen peroxide determination; Methods. Procedures. Technologies; Optical fibres; Sol–gel methods; Various methods and equipments; Horseradish peroxidase; Chemiluminescence; Sol–gel methods; Hydrogen peroxide determination; Optical fibres; Performance evaluation; Enzyme; Immobilization; Hydrogen Peroxides; Peroxidases; Sol gel process; Biosensor; Microencapsulation; Peroxidase; Oxidoreductases; Quantitative analysis; Optical fiber
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/S0003-2670(98)00080-4

Horseradish peroxidase (HRP) was immobilized by microencapsulation in sol–gel crystals derived from tetramethyl orthosilicate. A sol–gel biosensor based on the chemiluminescence reaction of the hydrogen peroxide–luminol–HRP system was developed. Calibration plots for hydrogen peroxide have been established by chemiluminescence measurements in a cuvette and through an optical fibre modified at its end with the immobilized HRP gel. The detection limit was 6.7×10 −4 M and the linear range was 0.1–3.0 mM. The relative standard deviation was 7.1% ( n=4). This method was applied to hydrogen peroxide determination in disinfectant solutions for contact lenses.