Genetic analysis of a local population of Oryza glumaepatula using SSR markers: implications for management and conservation programs

• Published in: Genetica Vol. 137; no. 2; pp. 221 - 231
• Main Authors: de Campos Vaz, Ana Rosa, de Oliveira Borba, Tereza Cristina, Brondani, Claudio, Rangel, Paulo Hideo Nakano, de Oliveira Camargo, Graziela Silvia, de Campos Telles, Mariana Pires, Filho, José Alexandre Felizola Diniz, Brondani, Rosana Pereira Vianello
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.11.2009; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Brazil; Cluster Analysis; Conservation of Natural Resources - methods; Evolutionary Biology; Gene Frequency; Genetic Markers - genetics; Genetic Variation; Genetics, Population; Human Genetics; Life Sciences; Local population; Microbial Genetics and Genomics; Minisatellite Repeats - genetics; Oryza - genetics; Oryza glumaepatula; Plant Genetics and Genomics; Quantitative Trait Loci - genetics; Rivers; Seed collection; Upstream; Brazil; Wild rice; Microsatellite markers; Paraguay River; Population genetics; Conservation genetics; Natural genetic resources
• ISSN: 0016-6707; 1573-6857
• DOI: 10.1007/s10709-009-9393-8

Knowledge of natural diversity and population structures of wild species, which might be related to cultivated species, is fundamental for conservation and breeding purposes. In this study, a genetic characterization of a large population of Oryza glumaepatula , occurring in a 10 km 2 area located at Tamengo Basin (Paraguay River, Brazil), was performed using SSR markers. This population is annually dragged from the river to permit navigation; one goal of this study was to examine the impact of this removal on genetic variability. From 18 polymorphic SSR markers, a total of 190 alleles were detected in a sample of 126 individuals, with an average of 10.3 alleles/locus, and a H e of 0.67. The five QTL-related markers showed an average H e value of 0.56, while the remaining 13 markers detected an average estimate of 0.70. An apparent outcrossing rate of 30%, a high proportion of alleles at low frequencies (56%), and the presence of exclusive alleles (9.5%) were found, with strong evidence of the establishment of individuals from different populations upstream in the Paraguay River. For conservation purposes, the river drag has no effect on the population. However, periodical seed collection from the Corumbá population can preserve part of the genetic variability present in upstream populations reducing the need for upriver collecting expeditions.