Strain Development and Medium Optimization for Fumaric Acid Production

• Published in: Biotechnology and bioprocess engineering Vol. 15; no. 5; pp. 761 - 769
• Main Authors: Kang, S.W., Korea University, Seoul, Republic of Korea, Lee, H.W., Kwangwoon University, Seoul, Republic of Korea, Kim, D.H., Kwangwoon University, Seoul, Republic of Korea, Lee, D.H., Korea Institute of Industrial Technology, Chonan, Republic of Korea, Kim, S.Y., Korea Institute of Industrial Technology, Chonan, Republic of Korea, Chun, G.T., Kangwon National University, Chuncheon, Republic of Korea, Lee, J.W., Sogang University, Seoul, Republic of Korea, Kim, S.W., Korea University, Seoul, Republic of Korea, Park, C.H., Kwangwoon University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society for Biotechnology and Bioengineering 01.10.2010; Springer Nature B.V; 한국생물공학회
• Subjects: Acid production; ACIDE FUMARIQUE; ACIDO FUMARICO; Acids; Bioreactors; Biotechnology; Catalytic oxidation; Chemistry; Chemistry and Materials Science; Engineering; Fermentation; FUMARIC ACID; gamma Radiation; Glucose; Hydrocarbons; Independent variables; Industrial and Production Engineering; MUTACION; Mutagenesis; MUTATION; Nitrogen; Nitrogen sources; Optimization; Optimization techniques; Organic nitrogen; Research Paper; response surface methodology; RHIZOPUS ORYZAE; Statistical methods; Studies; Variance analysis; 생물공학; fumaric acid; optimization; mutagenesis; response surface methodology (RSM)
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-010-0081-4

Rhizopus gate RUR709 mutant was isolated based on halo size from selection medium via mutagenesis with UV and γ-rays, and the production of fumaric acid in the submerged fermentation was assessed. The maximum concentration of fumaric acid was obtained using 0.5% corn steep liquor (CSL) as the nitrogen source. Organic nitrogen sources were shown to be more effective in fumaric acid production than inorganic nitrogen sources. Using optimum medium obtained by response surface methodology (RSM), the maximum concentration of fumaric acid achieved in flask culture was 26.2 g/L, which is fairly close to the 27.4 g/L predicted by the model. The highest concentration of fumaric acid in the stirred-tank reactor generated by the R. oryzae RUR709 mutant was 32.1 g/L and yield (0.45 g/g) and productivity (0.32 g/L/h) were highest at 4 days.