sensitive immunochromatographic assay using colloidal gold-antibody probe for the rapid detection of semicarbazide in meat specimens

• Published in: European food research & technology Vol. 232; no. 1; pp. 9 - 16
• Main Authors: Tang, Yong, Xu, Jinting, Wang, Wuzhou, Xiang, Junjian, Yang, Hongyu
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 2011; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Agriculture; Analysis; Analytical Chemistry; Antibiotics; Antibody; Bans; Biological and medical sciences; Biotechnology; bovine serum albumin; Chemistry; Chemistry and Materials Science; Chromatography; Colloidal gold particle; Competition; detection limit; enzyme-linked immunosorbent assay; Enzymes; eyes; Food; Food industries; Food processing industry; Food Science; Forestry; Fundamental and applied biological sciences. Psychology; General aspects; Gold; immunoaffinity chromatography; Immunochromatographic assay; Immunology; Laboratory animals; Mass spectrometry; Meat; Meat and meat product industries; Meat products; Metabolites; Methods of analysis, processing and quality control, regulation, standards; Monoclonal antibodies; Original Paper; Physiology; pork; Proteins; Scientific imaging; Semicarbazide; Sodium; Studies; temperature; Antibody; Colloidal gold particle; Semicarbazide; Immunochromatographic assay; Gold; Immunoaffinity chromatography; Meat product; Probe; Meat; Particle; Analysis method; Control method; Detection
• ISSN: 1438-2377; 1438-2385
• DOI: 10.1007/s00217-010-1351-2

A rapid immunochromatographic assay (ICA) was developed for the detection of semicarbazide (SEM), a nitrofurazone metabolite in meat specimens. Colloidal gold-labeled monoclonal antibody specific to a SEM derivative, 4[(4-carboxyphenyl)methylene]-hydrazinecarboxamide (CPSEM), was used as a probe for the presence of SEM. The assay is based upon the competitive reactivity theory that SEM derivatized with 4-carboxybenzaldehyde (4-CBA) competes with CPSEM-conjugated bovine serum albumin for binding the colloidal gold-labeled monoclonal antibody, the result of which could be read directly by naked eyes. Under optimal conditions, the visual detection limit is 0.72 ng mL⁻¹, which demonstrated the high sensitivity of this assay. The stability test indicated that the immunochromatographic strips could be used within 7 weeks at room temperature without significant loss of activity. Spiked pork samples were detected by ICA and confirmed by enzyme-linked immunosorbent assay (ELISA). The assay time for SEM detection was within 5 min, suitable for a rapid on-site testing for meat samples.