Polysaccharides purified from Cordyceps cicadae protects PC12 cells against glutamate-induced oxidative damage

• Published in: Carbohydrate polymers Vol. 153; pp. 187 - 195
• Main Authors: Olatunji, Opeyemi J., Feng, Yan, Olatunji, Oyenike O., Tang, Jian, Wei, Yuan, Ouyang, Zhen, Su, Zhaoliang
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 20.11.2016
• Subjects: Cordyceps cicadae; Glutamate; Neuroprotection; PC12 cells; Polysaccharide; Neuroprotection; Glutamate; Polysaccharide; PC12 cells; Cordyceps cicadae
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2016.06.108

•Two polysaccharides CPA-1 and CPB-2 were purified from Cordyceps cicadae.•CPA-1 and CPB-2 protected PC12 cells from glutamate induced toxicity by increasing cell survival rate, decreasing the accumulation of ROS and Ca2+.•CPA-1 and CPB-2 increased GSH-Px and SOD activites. Two polysaccharides CPA-1 and CPB-2 were isolated purified from Cordyceps cicadae by hot water extraction, ethanol precipitation and purification using anion exchange and gel filtration chromatography. Preliminary structural characterization of CPA-1 and CPB-2 were performed. The protective effect of CPA-1 and CPB-2 against glutamate-induced oxidative toxicity in PC12 cells was analyzed. The results indicated that pretreatment of PC12 cells with CPA-1 and CPB-2 significantly increased cell survival, Ca2+ overload and ROS generation. CPA-1 and CPB-2 also markedly up-regulated the antioxidant status of pretreated PC12 cells. Our results suggested that Cordyceps cicadae polysaccharides can protect PC12 cells against glutamate excitotoxicity and might serve as therapeutic agents for neuronal disorders.