Novel synthesized zinc oxide nanoparticles loaded alginate-chitosan biofilm to enhanced wound site activity and anti-septic abilities for the management of complicated abdominal wound dehiscence

• Published in: Journal of photochemistry and photobiology. B, Biology Vol. 192; pp. 124 - 130
• Main Authors: Gong, Cui-Ping, Luo, Ying, Pan, Ying-Ying
• Format: Journal Article
• Language: English
• Published: Switzerland Elsevier B.V 01.03.2019; Elsevier BV
• Subjects: Abdominal wound dehiscence; Alginates; Alginic acid; Antibacterial activity; Antimicrobial; Biocompatibility; Biocomposite; Biodegradability; Biodegradation; Biofilms; Biomedical materials; Biopolymers; Chitosan; Crystallites; Crystals; Dehiscence; Fourier transforms; Infrared spectroscopy; Line broadening; Microscopy; Morphology; Nanoparticles; Pore formation; Scanning electron microscopy; Spectroscopy; Spectrum analysis; Strong interactions (field theory); Surgery; Transmission electron microscopy; Wound healing; X-ray diffraction; Zinc; Zinc oxide; Zinc oxides; Nanoparticles; Antimicrobial; Wound healing; Biocomposite; Abdominal wound dehiscence
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2019.01.019

Wound dehiscence is a surgical complication and its management is inevitable because 25% to 35% of patients suffered from post laparotomy wound dehiscence. The excellent biodegradability and biocompatibility of chitosan and alginate have provided ample space for future developments in biomedical applications. Hence, the present work is directed towards the synthesis of robust biofilm made up of chitosan (CS), zinc oxide (ZnO) nanoparticles and Alginate (Alg). Chitosan and alginate were used for their pore forming ability, and ZnO is for its antibacterial action. The proposed biofilm was characterized with different characterization techniques such as Fourier Transform Infrared (FTIR) spectroscopy, UV–vis spectroscopy, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Transmission Electron microscopy (TEM) analyses. FTIR results inferred the strong interaction between the three components. The surface morphology of ZnO-CS/Alg. biofilm was exhibited as the spherical shaped nanoparticles which are firmly anchored on the polymer matrix. TEM analysis also confirmed the formation of biofilm. The XRD analysis confirmed the presence of ZnO in the biopolymer. The line broadening suggests that the crystallize size is in few nanometers. The average crystallite size was estimated as 50 nm using Scherrer formula. The antibacterial activity of the biofilm was successfully established against bacterial pathogens. Therefore, the developed materials have a potential play as antimicrobial role for the abdominal wound healing and biomedical fields. [Display omitted] •Synthesis of robust biofilm made up of chitosan, ZnO nanoparticles and Alignate•Biopolymers were used for their pore forming ability, and ZnO is for its antibacterial action.•Microscopic and spectroscopic analysis also confirmed the formation of biofilm.•Developed materials have played for antimicrobial and abdominal wound site healing.