Enzyme inactivation in serum before determination of total bile acids

Endogenous NADH-generating enzymes must be inactivated before total serum bile acids can be measured accurately by the direct enzymic method. To do this, we pretreat the sera with NaOH, in a final concentration of 0.1 mol/L. Consequently, lactate dehydrogenase activity at least as high as 30 000 U/L...

Full description

Saved in:
Bibliographic Details
Published inClinical chemistry (Baltimore, Md.) Vol. 29; no. 6; pp. 1073 - 1075
Main Authors Hanson, NQ, Freier, EF
Format Journal Article
LanguageEnglish
Published Washington, DC Am Assoc Clin Chem 01.06.1983
American Association for Clinical Chemistry
Subjects
Analytical, structural and metabolic biochemistry
Bile Acids and Salts - blood
Biological and medical sciences
Enzyme Activation - drug effects
Enzymes - blood
Fundamental and applied biological sciences. Psychology
Humans
Methods
NAD - metabolism
Other biological molecules
Sodium Hydroxide
Terpenes, steroids. Hormones
Online AccessGet full text

Cover

More Information
Summary:Endogenous NADH-generating enzymes must be inactivated before total serum bile acids can be measured accurately by the direct enzymic method. To do this, we pretreat the sera with NaOH, in a final concentration of 0.1 mol/L. Consequently, lactate dehydrogenase activity at least as high as 30 000 U/L is destroyed, obviating blank determinations. Values for bile acid in serum, so obtained, agree with values obtained after pretreatment with heat, an alkali-methanol solution, or sodium pyruvate, but our pretreatment has the advantages of ease, speed, economy, and negligible blank values.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/29.6.1073