Callus Culture of Sainfoin (Onobrychis viciifolia) and Plant Regeneration through Somatic Embryogenesis

Callus of sainfoin (Onobrychis viciifolia Scop.) was initiated from stem and root explants which were obtained from seedlings growing in vitro, on Linsmaier Skoog (LS) medium supplemented with 1 mg l−1 2, 4-D and 1 mg l−1 BA or only 1 mg l−1 BA, and the Vacin and Went medium without hormones. Somati...

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Bibliographic Details
Published inAnnals of botany Vol. 60; no. 3; pp. 309 - 313
Main Author GU, ZHUPING
Format Journal Article
LanguageEnglish
Published London Oxford University Press 01.09.1987
Academic Press Inc
Academic Press
Subjects
Agronomy. Soil science and plant productions
Biological and medical sciences
Callus
callus culture
Economic plant physiology
Embryos
Fundamental and applied biological sciences. Psychology
Hormones
In vitro culture
Liquids
Onobrychis viciifolia
Plant physiology and development
plant regeneration
Plantlets
Plants
Solids
Somatic embryogenesis
Somatic embryos
Subcultures
Tissue cultures, protoplasts
Embryonic development
Regeneration
Onobrychis sativa
Leguminosae
Dicotyledones
Angiospermae
Tissue culture
Spermatophyta
Fodder crop
Somatic embryo
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Summary:Callus of sainfoin (Onobrychis viciifolia Scop.) was initiated from stem and root explants which were obtained from seedlings growing in vitro, on Linsmaier Skoog (LS) medium supplemented with 1 mg l−1 2, 4-D and 1 mg l−1 BA or only 1 mg l−1 BA, and the Vacin and Went medium without hormones. Somatic embryos were formed on LS medium containing 1 m l−1 BA. Embryos developed into complete plants on filter paper saturated with hormone-free LS medium.
Bibliography:Present address: Department of Biology, Lanzhou University, Lanzhou, The People's Republic of China.
istex:0587013B715C44D2D83DFFB523A1206BFD4F8244
ark:/67375/HXZ-M2B475C7-7
ArticleID:60.3.309
ISSN:0305-7364
1095-8290
DOI:10.1093/oxfordjournals.aob.a087449