plant nuclear envelope protein MAF1 has an additional location at the Golgi and binds to a novel Golgi-associated coiled-coil protein

• Published in: Planta Vol. 222; no. 6; pp. 1028 - 1040
• Main Authors: Patel, S, Brkljacic, J, Gindullis, F, Rose, A, Meier, I
• Format: Journal Article
• Language: English
• Published: Berlin Springer-Verlag 01.12.2005; Springer; Springer Nature B.V
• Subjects: Amino Acid Sequence; amino acid sequences; Amino acids; Antibodies; Arabidopsis Proteins - chemistry; Arabidopsis Proteins - metabolism; Biological and medical sciences; Blotting, Western; Cell biochemistry; Cell Division; Cell lines; Cell physiology; Cellular biology; conserved sequences; Fluorescence; Fundamental and applied biological sciences. Psychology; Golgi apparatus; Golgi Apparatus - metabolism; green fluorescent protein; Green Fluorescent Proteins - analysis; GTPase-Activating Proteins - metabolism; Matrix Attachment Region Binding Proteins - metabolism; membrane proteins; Membrane Proteins - chemistry; Membrane Proteins - metabolism; Microscopy, Confocal; Molecular Sequence Data; Mutagenesis, Insertional; Nicotiana - cytology; Nuclear Envelope - metabolism; Nuclear membrane; nucleotide sequences; plant biochemistry; Plant cells; Plant physiology and development; plant proteins; Plant Proteins - metabolism; Protein Binding; Protein Interaction Mapping; Protein Structure, Tertiary; Protein Transport; protein-protein interactions; Proteins; recombinant fusion proteins; Solanum lycopersicum - metabolism; Solanum lycopersicum var. lycopersicum; Tomatoes; Two-Hybrid System Techniques; vegetable crops; WPP domain containing protein; Yeasts; Arabidopsis; WAP; Cell division; Blue-native gel electrophoresis; Coiled-coil; Golgi apparatus; Cruciferae; Dicotyledones; Angiospermae; Lycopersicon esculentum; Spermatophyta; Golgi; Solanaceae; MAF1; WPP domain; Cytoplasm; Golgin
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-005-0076-0

Tomato MAF1 (LeMAF1) is a plant-specific, nuclear envelope (NE)-associated protein. It is the founding member of a group of WPP domain-containing, NE-associated proteins. This group includes the Arabidopsis WPP family, which is involved in cell division, as well as plant RanGAPs. In addition to its NE localization, LeMAF1 accumulates in speckles in the cytoplasm. Here, we show that the LeMAF1-containing speckles are components of the Golgi apparatus. A novel tomato coiled-coil protein was identified that specifically binds to LeMAF1. Tomato WPP domain-associated protein (LeWAP) interacts in yeast and in vitro through its coiled-coil domain with several WPP-domain containing proteins, including AtRanGAP1 and the WPP family (LeMAF, WPP1 and WPP2). Like LeMAF1, LeWAP is localized at the Golgi. Moreover, we present data showing that Arabidopsis WAP is necessary for the existence of a multi-protein complex containing WPP2.