Expression of PTCH gene in ameloblastoma and odontogenic keratocyst: A comparative study

• Published in: Journal of oral and maxillofacial pathology : JOMFP Vol. 27; no. 2; pp. 427 - 428
• Main Authors: Srinagesh, Sharada, Sahana, N, Suganya, G, Renuga, S, Natarajan, Srikant, Kulkarni, Meghashyama
• Format: Journal Article
• Language: English
• Published: Chennai Wolters Kluwer India Pvt. Ltd 01.04.2023; Medknow Publications and Media Pvt. Ltd; Medknow Publications & Media Pvt. Ltd; Wolters Kluwer - Medknow
• Subjects: Ameloblastoma; Comparative analysis; Cross-sectional studies; Gene expression; Genes; Genetic research; Hedgehog protein; Original; Patched protein; Patched1 gene; Statistical analysis; Therapeutic targets; Ameloblastoma; mutation; PTCH; odontogenic keratocyst
• ISSN: 0973-029X; 1998-393X
• DOI: 10.4103/jomfp.jomfp_198_22

Aim: This is a cross-sectional comparative study, aimed to quantify the expression of patched (PTCH) gene in ameloblastoma, odontogenic keratocyst (OKC) and also the comparison of both the expressions. Materials and Methods: Genomic deoxyribonucleic acid (DNA) was extracted and quantified, and the expression of the PTCH gene was done in 17 cases of ameloblastoma and 17 cases of OKC by quantitative real-time polymerase chain reaction (RT-qPCR). Results: It was observed that there was an overexpression of the PTCH gene in both ameloblastoma and OKC with a good mean cycle threshold (CT) value of 32.71 ± 2.432 and 34.69 ± 1.875, respectively. When comparing the PTCH expression between the two, ameloblastoma showed higher expression than the OKC and the difference is statistically significant with P value of 0.025. Conclusions: Our findings suggest that there is overexpression of PTCH in ameloblastoma and OKC, but it is highly expressed in ameloblastoma when compared to OKC. Overexpression of PTCH may constitute the activation of the Sonic Hedgehog pathway and may suggest the mechanism for the development of ameloblastoma and OKC. Hence it can be used as a valuable marker for early diagnosis and in the identification of therapeutic targets.