Evaluation of 1-ethyl-3-methylimidazolium acetate based ionic liquid systems as a suitable solvent for collagen
ABSTRACT Collagen, a prominent biopolymer, which is famous for its excellent biological activity, has been used extensively for tissue engineering applications. In this study, a novel solvent system for collagen was developed with an ionic liquid, 1‐ethyl‐3‐methylimidazolium acetate ([EMIM][Ac]), so...
Saved in:
Published in | Journal of applied polymer science Vol. 130; no. 4; pp. 2245 - 2256 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken, NJ
Blackwell Publishing Ltd
15.11.2013
Wiley Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | ABSTRACT
Collagen, a prominent biopolymer, which is famous for its excellent biological activity, has been used extensively for tissue engineering applications. In this study, a novel solvent system for collagen was developed with an ionic liquid, 1‐ethyl‐3‐methylimidazolium acetate ([EMIM][Ac]), solvent system. A series of sodium salts were introduced into this solvent system to enhance collagen's dissolution procedure. The results show that the solubility of collagen was significantly influenced by the temperature and sodium salts. The solubility reached up to approximately 11% in the [EMIM][Ac]/Na2HPO4 system at 45°C. However, the structure of the regenerated collagen (Col‐regenerated) may have been damaged. Hence, we focused on the structural integrity of the collagen regenerated from the [EMIM][Ac] solvent system by the methods of sodium dodecyl sulfate–polyacrylamide gel electrophoresis, Fourier transform infrared spectroscopy, ultrasensitive differential scanning calorimetry, atomic force microscopy, X‐ray diffraction, and circular dichroism because its signature biological and physicochemical properties were based on its structural integrity. Meanwhile, a possible dissolution mechanism was proposed. The results show that the triple‐helical structure of collagen regenerated from the [EMIM][Ac] solvent system below 35°C was retained to a large extent. The biocompatibility of Col‐regenerated was first characterized with a fibroblast adhesion and proliferation model. It showed that the Col‐regenerated had almost the same good biological activity as nature collagen, and this indicated the potential application of [EMIM][Ac] in tissue engineering. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 2245–2256, 2013 |
---|---|
Bibliography: | International Scientific and Technological Cooperation Project - No. SH201108 ark:/67375/WNG-J6944ZZP-6 National Natural Science Foundation of China - No. 21276164 Science and Technology Planning Project of China - No. 2011BAC06B11 istex:A27B9EECF221D68FDF5372DEEA398228A9918FC9 ArticleID:APP39298 Science and Technology Planning Project of Chengdu - No. 10GGYB345SW-023 |
ISSN: | 0021-8995 1097-4628 |
DOI: | 10.1002/app.39298 |