Differentiation of closely related Globodera pallida (Stone) populations by means of DNA restriction fragment length polymorphisms (RFLPs)

• Published in: Journal of phytopathology Vol. 130; no. 2; pp. 127 - 136
• Main Authors: Schnick, D. (Biologische Bundesanstalt fuer Land- und Forstwirtschaft, Braunschweig (Germany, F.R.). Inst. Biochemie), Rumpenhorst, H.J, Burgermeister, W
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.10.1990; Blackwell
• Subjects: ADN; Biological and medical sciences; Biotechnology; Chromatographie; DNS; Fundamental and applied biological sciences. Psychology; GLOBODERA; Globodera pallida; IDENTIFICACION; IDENTIFICATION; Identifizierung; Invertebrates; Nemathelminthia. Plathelmintha; Nematodes; Nukleinstoff; Correlation; Biochemical analysis; Typing; Genetic marker; Virulence; Genetic distance; Globodera pallida; Pest; Restriction fragment length polymorphism; DNA; Pathotype; Helmintha; Population; Nemathelminthia; Molecular probe; Invertebrata; Differentiation; Nematoda; Nucleic acid
• ISSN: 0931-1785; 1439-0434
• DOI: 10.1111/j.1439-0434.1990.tb01160.x

A small genomic library from DNA of Globodera pallida population Delmsen (pathotype 3) was constructed. A number of clones were tested as hybridization probes with 21 G. pallida and 6 G. rostochiensis populations. The two species were easily distinguished by their hybridization patterns. A total of 32 RFLP markers were identified which permitted the differentiation of all G. pallida populations. By means of cluster analysis of the RFLP data the genetic distances between the 21 G. pallida populations were calculated and a dendrogram was constructed. The arrangement of the 21 G. pallida populations in the dendrogram suggests a partial correlation between genetic distance and virulence according to biological pathotyping