An Evaluation of T‐Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation
Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically e...
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| Published in | Cytometry. Part A Vol. 97; no. 2; pp. 171 - 183 |
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| Main Authors | , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Hoboken, USA
John Wiley & Sons, Inc
01.02.2020
Wiley Subscription Services, Inc |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1552-4922 1552-4930 1552-4930 |
| DOI | 10.1002/cyto.a.23964 |
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| Abstract | Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter‐induced physical forces. In summary, flow cytometry cell‐sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. |
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| AbstractList | Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry.Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter-induced physical forces. In summary, flow cytometry cell-sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. Cell alterations during isolation and preparation for flow cytometry cell sorting by antibodies, temperature, homogenization, buffer composition and mitogens are well known. In contrast, little is known about cell alteration caused by the instrument or the sorting process itself. We systematically evaluated cellular responses to different sorter‐induced physical forces. In summary, flow cytometry cell‐sorting induced forces can affect cellular signaling cascades, especially the MAPK p38. Functional assays, related to the p38 MAPK pathway, of human primary T cells after flow cytometry sorting did lead to minor physiological modulation but no functional impairments. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. |
| Author | Andrä, Immanuel Schiemann, Matthias Przibilla, Sabine Stadler, Herbert Busch, Dirk H. Henkel, Lynette Angerpointner, Corinne Ritter, Julia Effenberger, Manuel Ulrich, Hanna Soll, Dominik Dürr, Susi |
| Author_xml | – sequence: 1 givenname: Immanuel surname: Andrä fullname: Andrä, Immanuel organization: Technische Universität München (TUM) – sequence: 2 givenname: Hanna surname: Ulrich fullname: Ulrich, Hanna organization: Universität zu Lübeck – sequence: 3 givenname: Susi surname: Dürr fullname: Dürr, Susi organization: Technische Universität München (TUM) – sequence: 4 givenname: Dominik surname: Soll fullname: Soll, Dominik organization: Technische Universität München (TUM) – sequence: 5 givenname: Lynette surname: Henkel fullname: Henkel, Lynette organization: Technische Universität München (TUM) – sequence: 6 givenname: Corinne surname: Angerpointner fullname: Angerpointner, Corinne organization: Technische Universität München (TUM) – sequence: 7 givenname: Julia surname: Ritter fullname: Ritter, Julia organization: Universität zu Lübeck – sequence: 8 givenname: Sabine surname: Przibilla fullname: Przibilla, Sabine organization: Juno Therapeutics GmbH – sequence: 9 givenname: Herbert surname: Stadler fullname: Stadler, Herbert organization: IBA GmbH, IBA Lifesciences – sequence: 10 givenname: Manuel surname: Effenberger fullname: Effenberger, Manuel organization: Technische Universität München (TUM) – sequence: 11 givenname: Dirk H. orcidid: 0000-0001-8713-093X surname: Busch fullname: Busch, Dirk H. organization: National Centre for Infection Research (DZIF) – sequence: 12 givenname: Matthias surname: Schiemann fullname: Schiemann, Matthias email: matthias.schiemann@tum.de organization: Technische Universität München (TUM) |
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| Keywords | traceless affinity cell selection cell sorting cell stress cell purification MAPK T cells flow cytometry cell sorting p38 activation |
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| SubjectTerms | Antibodies Cell activation cell purification cell sorting cell stress Flow cytometry flow cytometry cell sorting Lymphocytes Lymphocytes T MAP kinase MAPK Mitogens p38 activation T cells traceless affinity cell selection |
| Title | An Evaluation of T‐Cell Functionality After Flow Cytometry Sorting Revealed p38 MAPK Activation |
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