Human brain endothelial cells and astrocytes produce IL-1 beta but not IL-10

• Published in: Scandinavian journal of immunology Vol. 44; no. 5; p. 506
• Main Authors: Corsini, E, Dufour, A, Ciusani, E, Gelati, M, Frigerio, S, Gritti, A, Cajola, L, Mancardi, G L, Massa, G, Salmaggi, A
• Format: Journal Article
• Language: English
• Published: England 01.11.1996
• Subjects: Adult; Astrocytes - immunology; Astrocytes - metabolism; beta 2-Microglobulin - biosynthesis; beta 2-Microglobulin - genetics; Brain - blood supply; Brain - pathology; Capillaries - cytology; Capillaries - metabolism; Cells, Cultured; Endothelium, Vascular - metabolism; Female; Gene Expression; Humans; Infant, Newborn; Interleukin-1 - biosynthesis; Interleukin-1 - genetics; Interleukin-10 - biosynthesis; Interleukin-10 - genetics; Intracranial Aneurysm - metabolism; Intracranial Aneurysm - pathology; Male; Meningeal Neoplasms - metabolism; Meningeal Neoplasms - pathology; Meningioma - metabolism; Meningioma - pathology; Middle Aged; Multiple Sclerosis - metabolism; Multiple Sclerosis - pathology; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Tumor Necrosis Factor-alpha - biosynthesis; Tumor Necrosis Factor-alpha - genetics; Umbilical Veins - cytology; Umbilical Veins - metabolism
• ISSN: 0300-9475
• DOI: 10.1046/j.1365-3083.1996.d01-343.x

The ability of human brain endothelial cells to produce mRNA for interleukin-10, and release IL-10 in culture supernatants after in vitro stimulation with LPS, TNF-alpha and gamma-IFN was assessed and compared to that of astrocytes, peripheral blood mononuclear cells and human umbilical vein endothelial cells. IL-1 beta and beta 2-microglobulin release were also analysed. IL-10 and TNF-alpha mRNA presence was investigated in normal brain as well as in three plaques from two multiple sclerosis patients. While increased IL-1 beta and beta 2-microglobulin release in the supernatants of stimulated cells could be detected in all the studied cell lineages, IL-10 mRNA and protein release was only seen in LPS-stimulated PBMNCs. Similarly, mRNA for IL-10 was not detected in CNS tissues, while TNF-alpha was present in all plaques. The lack of production of significant amounts of IL-10 by astrocytes and human brain endothelial cells suggests that these cells may not be the primary source of in vivo IL-10-mediated down-regulation of immune reactions within the central nervous system.