Influence of one selected Tisochrysis lutea strain rich in lipids on Crassostrea gigas larval development and biochemical composition

• Published in: Aquaculture nutrition Vol. 22; no. 4; pp. 813 - 836
• Main Authors: Da Costa, F., Petton, B., Mingant, C., Bougaran, G., Rouxel, C., Quéré, C., Wikfors, G.H., Soudant, P., Robert, R.
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell Publishing Ltd 01.08.2016; Hindawi Limited; Wiley
• Subjects: Agricultural sciences; Animal biology; Bacillariophyceae; Bivalvia; Chaetoceros neogracile; Crassostrea gigas; Food and Nutrition; Invertebrate Zoology; larvae; Life Sciences; lipids; Marine; oyster; Tisochrysis lutea; Zootechny; oyster; lipids; ACL; larvae; Tisochrysis lutea
• ISSN: 1353-5773; 1365-2095
• DOI: 10.1111/anu.12301

Effects of a remarkably high overall lipid Tisochrysis lutea strain (T+) upon gross biochemical composition, fatty acid (FA), sterol and lipid class composition of Crassostrea gigas larvae were evaluated and compared with a normal strain of Tisochrysis lutea (T) and the diatom Chaetoceros neogracile (Cg). In a first experiment, the influence of different single diets (T, T+ and Cg) and a bispecific diet (TCg) was studied, whereas, effects of monospecific diets (T and T+) and bispecific diets (TCg and T+Cg) were evaluated in a second experiment. The strain T+ was very rich in triglycerides (TAG: 93–95% of total neutral lipids), saturated FA (45%), monounsaturated FA (31–33%) and total fatty acids (4.0–4.7 pg cell−1). Larval oyster survival and growth rate were positively correlated with 18:1n‐7 and 20:1n‐7, in storage lipids (SL), and negatively related to 14:0, 18:1n‐9, 20:1n‐9, 20:4n‐6 and trans‐22‐dehydrocholesterol in membrane lipids (ML). Surprisingly, only the essential fatty acid 20:5n‐3 in SL was correlated positively with larval survival. Correlations suggest that physiological disruption by overabundance of TAG, FFA and certain fatty acids in larvae fed T+ was largely responsible for the poor performance of these larvae. ‘High‐lipid’ strains of microalgae, without regard to qualitative lipid composition, do not always improve bivalve larval performance.