Gene II of Phage f1: Its Functions and Its Products

Plasmids harboring the amino-terminal part of bacteriophage f1 gene II confer to bacterial cells partial resistance to infection with the male-specific bacteriophages f1 and f2. This effect (IP-2 phenotype) is due to the production of large amounts of a ≈ 20,000-dalton polypeptide corresponding to t...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 78; no. 9; pp. 5421 - 5424
Main Authors Dotto, Gian Paolo, Enea, Vincenzo, Zinder, Norton D.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.09.1981
National Acad Sciences
Subjects
Bacteriophages
Cell growth
Chimeras
Cloning, Molecular - methods
Coliphages - genetics
DNA
DNA, Superhelical - metabolism
DNA, Viral - biosynthesis
Endonucleases - genetics
Gels
Genes
Genes, Viral
Infections
Molecules
phage f1
Phenotypes
Plasmids
Viral Proteins - genetics
Virology
Virus Replication
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Summary:Plasmids harboring the amino-terminal part of bacteriophage f1 gene II confer to bacterial cells partial resistance to infection with the male-specific bacteriophages f1 and f2. This effect (IP-2 phenotype) is due to the production of large amounts of a ≈ 20,000-dalton polypeptide corresponding to the amino-terminal part of gene II protein. These results have allowed the isolation of clones producing functional gene II protein in large amounts. An in vitro assay has been developed to test the enzymatic activity of the gene II protein produced.
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ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.78.9.5421