Analysis of glutathione by capillary electrophoresis based on sample stacking
Glutathione is a small peptide, which participates in cellular oxidation‐reduction and detoxification. It is present in most biological tissues at different concentrations. The oxidized and reduced forms of the peptide were measured in erythrocytes and myocardial tissue by capillary electrophoresis...
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Published in | Electrophoresis Vol. 22; no. 11; pp. 2351 - 2354 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.07.2001
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Subjects | |
Online Access | Get full text |
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Summary: | Glutathione is a small peptide, which participates in cellular oxidation‐reduction and detoxification. It is present in most biological tissues at different concentrations. The oxidized and reduced forms of the peptide were measured in erythrocytes and myocardial tissue by capillary electrophoresis based on stacking. After tissue homogenization or hemolysis of the red blood cells, the samples were deproteinized with acetonitrile and injected filling about 13% of the capillary volume. The electrophoresis was performed at 10 kV using a separation buffer of 250 mM borate, 50 mM Tris, pH 8.0. Sample stacking increased the sensitivity of detection by 10–20‐fold. |
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Bibliography: | ark:/67375/WNG-H7BJ7188-6 istex:B8541C5FE1668CD0768B05E48037E03591F0C022 ArticleID:ELPS2351 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/1522-2683(20017)22:11<2351::AID-ELPS2351>3.0.CO;2-C |